COMPREHENSIVE APPROACHES TO THE CAPTURE, IDENTIFICATION. AND QUANTITATIVE ANALYSIS OF ANTIMICROBIAL PEPTIDES AND OTHER PEPTIDES OF INTEREST FROM BIOLOGICAL SOURCES
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Date
2024
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Saudi Digital Library
Abstract
The emergence of antibiotic-resistant pathogens necessitates the development of novel antimicrobial agents. This dissertation focuses on the synthesis and characterization of magnetic iron (III) oxide particles (IOPs) incorporating amphipathic cross-linked polymers, specifically N-methacryloyl-6-aminohexanoic acid (MA6AHA) and N-isopropylacrylamide/methacrylic acid (NIPMAm/MAA). These IOPs are evaluated for their stability and effectiveness in capturing antimicrobial peptides (AMPs) from biological sources, using American alligator plasma as a model system. The captured peptides were analyzed using tandem mass spectrometry (LC-MS/MS), employing electron-transfer/higher-energy collision dissociation (EThcD) fragmentation techniques, and data analysis was conducted with PEAKS Xpro software. Results demonstrated that the functionalized IOPs efficiently captured a variety of peptides, including potential AMPs, highlighting their potential utility in discovering novel bioactive peptides. This study contributes to the broader research effort in combating antibiotic resistance and exploring the reptilian host-peptidome.
Description
This dissertation work was conducted under the supervision of Dr. Barney Bishop at George Mason University and was submitted in fulfillment of the requirements for the Ph.D. degree in Chemistry and Biochemistry. The work includes experimental proteomics, peptide extraction methods, LC-MS/MS analysis, and data interpretation using PEAKS Xpro. The deposited file contains the full dissertation as approved by the examining committee in 2024.
Keywords
Antimicrobial peptides, Proteomics, Xenopus laevis, LC-MS/MS, IOP nanoparticles, C18 enrichment, Bioactive peptides