OlafSAHAR FARHAN H ALSHAMMARI2022-05-282022-05-28https://drepo.sdl.edu.sa/handle/20.500.14154/37393FRET mechanism was prescribed before few decades. Now, it’s being used mostly in medical science, drug development and pharmaceutical. Its process is dependent on the radiation-free energy conversion between two light responsive chromophores. A donor which is in electronically exited state, passes energy to an acceptor chromophore, by non-radiative dipole-dipole coupling. In FRET, energy is transferred without molecular collision and conversion to thermal energy. The energy conversion leads to a decrease in the fluorescence intensity of the donor and the lifetime of the higher energy level, and an increase in the emission intensity of the acceptor. I would use different references for the assessment of the FRET and through different parameters and frame of references due to surprising results which are based on instrument error and time-based issues.en