BOUCHET, SPINELLI AurélieBAWAZEER, Bassma2026-03-172024https://hdl.handle.net/20.500.14154/78453Biosensors are becoming a vital part of the future of medicine, especially for the early detection and monitoring of various molecules such as viruses, enzymes, and cells. The key advantage of biosensors is their ability to harness exciting biological processes to identify specific biological elements, like using sugars to capture glucosidase enzymes. In this study, we explore the use of a relatively new class of molecules known as iminosugars to detect glucosidases. Iminosugars have established their significance in the medical field and are found in drugs like Miglitol and Miglustat, which are used to treat diabetes and Gaucher’s disease. Their value lies in their unique interaction with glycosidases—the enzymes that catalyze the hydrolysis of oligosaccharides—without being consumed or cleaved in the process. This property makes iminosugars particularly valuable as bioreceptors, with potential applications in both medical diagnostics and the technological development of biosensors. This work is a continuation of Thibault Rancon’s PhD project, under the supervision of Dr. S. Py at DCM and Dr. A. Bouchet-Spinelli at SyMMES, The key goal of his thesis is to explore the design and synthesis of new iminosugars with enhanced activity and selectivity. He and Dr.Py developed iminosugars that will be utilized in creating biosensors and using Surface Plasmon Resonance imaging (SPRi) to analyze the interactions with glycosidases. This work aims to achieve the immobilization and identification of glycosidases in biological samples.29enBiosensorsenzymesSPRiThesis