Browsing by Author "ALKhalifah, Zahra Abdul Jaleel"

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The Cellular and Molecular Impacts of Doxorubicin on Breast and Ovarian Cancer Cells
(2022) ALKhalifah, Zahra Abdul Jaleel; ثابت، حسين حسن; المفتي، سارة أمين
Cancer is the most serious disease caused by gene mutation, these mutations can disturb three types of genes, DNA repair genes, proto-oncogenes, and tumor suppressor genes, which affect the cell cycle, proliferation, differentiation, and programmed cell death. Breast and ovarian cancer are the further most commonly occurring cancer in women globally. As of 2021, an estimated 281,550 females will be diagnosed with breast cancer, and about 43,600 of these females will pass away from this disease. Breast cancer (BC) may begin in various parts of the breast, such as the ducts, the lobules, or the tissues in between that contain many genetic and epigenetic abnormalities. Ovarian cancer (OC) has a high death rate worldwide and it is considered the seventh most fatal cancer among women globally. Cancer therapy has different approaches, chemotherapy is one of the best approaches, which is used worldwide. Doxorubicin (DOX) is one of the most effective chemotherapeutic drugs in cancer therapy. In the present study, we evaluated the role of DOX in cell cycle regulation, proliferation, differentiation, and apoptosis on BC, OC, and control healthy cells of human foreskin fibroblast cells. DOX was used in different doses (1 µM, 2 µM, 4 µM) and 0.1% DMSO for the control treatment, DOX incubation time was 24 h and 48 h. We performed a cell viability assay, wound healing assay, and quantitative real-time PCR. Finally, we evaluated the gene expression and alterations of different cancer-related genes. Results obtained indicated that there was suppression in some cancer-related genes (NOTCH1, PDGFA, TMEM45A, KRAS, NRAS, LAMA4, PTEN, ALK, and BRAF) in the MCF-7 and SKOV-3 cells, which indicate that the DOX has a major effect on inhibiting cancer cells proliferation.
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