Browsing by Author "ALSharif, Abdulaziz Saleh"

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Detailed Analysis on Extended Spectrum Beta-Lactamase Producing Enterobacteriaceae in Madinah, Kingdom of Saudi Arabia
(Saudi Digital Library, 2020) ALSharif, Abdulaziz Saleh; Khadri, Habeeb
BACKGROUND: Enterobacteriaceae producing Extended-Spectrum β-Lactamases (ESBL) are a significant hindrance to hospital infection management. This paper characterizes the origin of the ESBL strains and determines the prevalence of antibiotic resistance patterns. Samples were taken from resident patients at Ohud Hospital in Saudi Arabia. OBJECTIVES: The current study aims to identify the ESBL frequency by traditional screening such as cephalosporin antibiotic susceptibility reduction to ESBLstrains by disc diffusion, followed by VITEK 2. Confirmatory test using DDST and E-test, then molecular characterization by PCR. METHODS: From July to November 2019, 137 non-duplicate Enterobacteriaceae strains were taken from clinical samples gathered from resident patients at Ohud Hospital in Saudi Arabia. Automation methods of Vitek-2 were used to analyze antibiotic susceptibility and bacterial species identification.. The phenotypic methods were confirmed with the disc diffusion synergy test, and the multiplex PCR was used to detect gene prevalence of ESBL. RESULTS: From the 137 samples of K. pneumonia and E. coli (Enterobacteriaceae family)) collected, the overall prevalence of Klebsiella pneumonia was 64 isolated and, Escherichia coli had 73 isolates. The ESBL strains of Escherichia coli were 20 samples (27%) and Klebsiella pneumonia 33 samples (51%). In females highest rate of ESBL 36 (67%) isolates were identified in comparison to malesmales 17 (32%) isolates were confirmed. ESBL prevalence was high in older patients (≥60 years of age). Out of 53 ESBL isolates, K. pneumonia and E. coli had different gene types. 24 isolates had more than one ESBL gene, 22 isolates had a single gene, and 7 isolates were negative for either blaTEM and blaSHV or blaOXA. CONCLUSION: Our study shows the high ESBL such as E. coli and K. pneumonia isolates from clinical specimens in Ohud Hospital Madinah, Saudi Arabia. In addition, we also report the presence of the blaTEM, blaSHV, and blaOXA genes in the isolates, which is an alarming spread of ESBL among patients.
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