Browsing by Author "Alomair, Munirah"

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The effect of components of electrospun material on S. aureus, P. aeruginosa and E. coli bacterial growth and biofilm formation
(Saudi Digital Library, 2023-11-28) Alomair, Munirah; Powell, Lydia
The prevalence of chronic wounds in immunocompromised patients has risen recently, leading to increased morbidity and mortality rates. The emergence of antibiotic resistance has necessitated novel technology for the treatment of these wounds. In order to provide a bespoke wound dressing for patients, Corryn Biotechnologies is developing an innovative device that electrospins a polymer solution directly onto a wound. The antibiotic activity of the electrospun components, namely dimethyl sulfoxide (DMSO), collagen 1, acetone and a non-disclosed polymer solution, were tested against gram-positive methicillin-resistant Staphylococcus aureus (MRSA 1004A) and gram-negative Pseudomonas aeruginosa PAO1 and Escherichia coli IR57 in both planktonic and biofilm assays to determine their effect on bacterial growth and biofilm formation. Minimum inhibitory concentration (MIC) assays were used to evaluate the effect of DMSO against MRSA 1004A, P. aeruginosa PAO1, and E. coli IR57, with resultant MICs of 25%, 6.25%, and 12.5%, respectively. The MICs of collagen 1 and acetone were >50% for both gram-positive and gram-negative bacteria. Confocal laser scanning microscopy (CLSM) revealed that DMSO significant inhibited the formation of MRSA 1004A biofilm biomass at both concentrations of 1/2 MIC DMSO (p<0.0001) and 1/4 MIC DMSO (p=0.0017), with increased cell death achieved with 1/2 MIC DMSO treatment (p=0.0003). The MRSA 1004A biofilm roughness significantly increased with 1/2 MIC DMSO (p=0.0008), but the average thickness area and average thickness biomass were significantly decreased (both p<0.0001) at 1/2 MIC DMSO treatment. The P. aeruginosa PAO1 biofilm biomass significantly increased with 1/2 MIC DMSO treatment (p=0.03), while cellular death decreased with 1/4 MIC DMSO treatment (p=0.038). The effects of the components of the electrospun solution against the most common strains of gram-positive and gram-negative bacteria in chronic wounds highlight the potential application of these components to form a bespoke wound dressing to reduce biofilm formation and aid in chronic wound healing.
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