Browsing by Author "Alotaibi, Omar"

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Discovery of Independent Suppressors in C. elegans During CMTR1 Knockdown: Insights from a Generic Screen
(University of Aberdeen, 2024) Alotaibi, Omar; Pettitt, Jonathan
In this study, we used C. elegans as a model organism to investigate the regulation and function of CMTR1 through a direct mutagenesis screen using ethyl methanesulfonate (EMS). Our goal was to identify suppressors of CMTR1 knockdown that could restore fertility, employing an auxin-inducible degradation (AID) system to target CMTR1. However, we anticipated that EMS mutagenesis would reveal faults in the AID system, particularly through TIR1 loss-of-function mutations, which inadvertently prevented CMTR1 degradation and allowed the variants to maintain fertility. Our results confirmed our hypothesis, showing that TIR1 loss-of-function mutations prevent the degradation of CMTR1, thereby allowing CMTR1 to persist and the variants to maintain fertility. Analysing the frequency and nature of these mutations will be essential for estimating the size of the genuine suppressor targets identified through EMS screening. This approach will help us gauge the extent and scope of the suppressors that can compensate for CMTR1 knockdown. In addition to these control-related findings, we successfully identified Suppressors of CMTR1 Knockdown (SOCK) that restored fertility through mutations in other proteins or regulatory sequences. These findings suggest a complex regulatory network involving CMTR1, highlighting its crucial function beyond immune recognition and opening new avenues for understanding the broader biological roles of 2'-O-ribose methylation. In conclusion, this research can provide a clearer understanding of the importance of cap 1 and 2'-O-ribose methylation in cellular activities, highlighting their crucial roles in preserving fertility
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Ethical conflict experienced as a critical care nurse during the COVID-19 pandemic: a systematic review
(Queen's University Belfast, 2024-04-30) Alotaibi, Omar; Alotaibi, Omar
Background The COVID-19 pandemic had a devastating effect on healthcare workers and healthcare systems at large. The first case of COVID-19 in the Kingdom of Saudi Arabia was reported on 2 March 2020 and, by 15 July 2021, the Ministry of Health had reported 8020 fatalities from the contagion. The COVID-19 pandemic laid bare the unpreparedness of healthcare systems across the world, and further led to a substantial shift in occupational risks and various stressors pertaining to critical care nursing. Objectives A PEO framework was used to develop the research question to investigate the ethical conflicts that intensive care nurses experienced during the COVID-19 pandemic, and their impact on nurses’ psychological wellbeing and job satisfaction. The study aimed to identify and describe common ethical conflicts faced by critical care nurses during the pandemic, their psychological impacts, and offer recommendations for healthcare and relevant policymakers. Search Strategy The study adopted a qualitative approach, and a systematic literature review was conducted on existing studies. An induction approach was used in respect to the sample size of the selected studies. A systematic search was conducted across three electronic databases including CINAHL Plus, PubMed, and MEDLINE ALL. Studies conducted prior to COVID-19, those conducted on healthcare providers other than intensive care nurses, and sources published in languages other than English were exempted from the study. Results The search produced 738 articles which were further evaluated against the inclusion and exclusion criteria. A total of 12 studies matched the inclusion criteria and met the CASP threshold for quality appraisal. Upon synthesis, changes in practice or work situations (n=7), lack of knowledge (n=3), an organisation’s inefficiency in supporting nurses (n=6), psychological wellbeing (n=7), and job satisfaction (n=4) were the main themes identified encapsulating ethical issues. Conclusion Nurses had to work under unfamiliar conditions typified by a high workload, prolonged use of PPE, and an increased demand for care in ICUs, hence the potentiality for ethical conflicts. Amid the construction of new ICU centres and an increasing workload, nurses had to deal with novel experiences such as adapting to collaboration with new workers, the role of constantly acting as a supporter, and complexities linked to working with non-healthcare professionals. A failure to highlight these complexities may cause critical care nurses to perceive them as routine. Increased isolation also had emotional ramifications which could affect job satisfaction levels among ICU nurses. Involvement in practice during COVID-19 led to depression, psychological distress, insomnia, and anxiety. The study also established that discrimination over the distribution of PPE, fatigue, and burnout had a profound effect on nurses’ job satisfaction levels. There is a need for interventions tailored towards increasing organisational support, improving nurses’ wellbeing, and building capacity. Organisations should also include nurses in the decision-making process, and consider both extrinsic and intrinsic factors of motivation to enhance job satisfaction levels.
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Waste Management Best Practices in the Marble Industry: A Single Case Study of a Company in Saudi Arabia
(Technological University Dublin, 2024-08-31) Alotaibi, Omar; Glynn, Gerard
impact on sustainability and environment. The research evaluates the processes in the production of marbles in the context of supply chain directing our research to a special case study of a company in Saudi Arabia. The focus is enhancing sustainability and minimise carbon footprint. Utilising both deductive and inductive approach, the study involves qualitative methods where the researcher will apply semi-structured interview techniques to gather data. Targeted interviewees range from top leadership to business level and at operational level where a total of 6 interviews will take place to gather insightful information. The data will apply thematic analysis to identify best practices and areas of improvements in managing waste with an additional triangulation and member checking to ensure data validity and reliability. The findings provided insights into our current waste management practices, revealing significant strategies to adapt and inefficiencies to keep away from. Implication of study reveals the necessity of acknowledging that waste management in the marble industry must become more comprehensive. Additionally, the study established that technological development is helpful in the improvement of sustainable development. Theoretically, this research adds knowledge and provides information about circular economy principles in the manufacturing industries to future researchers and the public.
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