Browsing by Author "Altayyar, Ahmed"

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Prevalence, antibiotic susceptibility, and characterization of Methicillin-resistant Staphylococcus aureus inpatients and healthcare workers at King Khalid Hospital, Hail, Kingdom of Saudi Arabia
(Saudi Digital Library, 2020) Altayyar, Ahmed; Khadri, Habeeb
BACKGROUND As a nosocomial pathogen, Methicillin-Resistant Staphylococcus aureus (MRSA) results in significant mortality and morbidity worldwide, as it emerged as one of the known causes of hospital-acquired infection and remains a significant contributor to management failure. Nasal carriage of Staphylococcus aureus (S. aureus) plays a significant role in the pathogenesis and epidemiology of infection. OBJECTIVES The current study aims to identify the carriers of MRSA prevalence in healthcare staff and patients, identify the inducible detection of clindamycin resistance, their susceptibility pattern to antibiotics, and compare molecular approaches with traditional methods for MRSA's rapid detecting. MATERIALS AND METHODS In this study, 748 nasal swabs were screened for MRSA from in-patients and 102 from healthcare workers. They were subjected to conventional culture and automation (Phoenix) for phenotypic determination, including antibiotic susceptibility testing, screening for cefoxitin, oxacillin, inducible clindamycin resistance, and PCR (Xpert) for genotypic determination. RESULTS The prevalence of MRSA among in-patients by PCR was 11.89% and by culture was 5.5%. In healthcare workers, it was 11.76% and 0%. Out of 110 positive MRSA cases, 62.70% were PCR positive, 8.20% were culture positive, and 29.10% were positive for both. 13.6% of MRSA was recovered from the pediatric ward, which had an age range of 0–10 years. Inducible resistance of clindamycin was around 21.95%, and vancomycin sensitivity was 100%. CONCLUSION MRSA colonization in healthcare staff might be an infection source, where Polymerase Chain Reaction (PCR) was concluded to be rapid and more sensitive than culture. Vancomycin sensitivity was 100% and could be applied for MRSA treatment in infected patients.
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