Browsing by Author "Assery, Nasser"

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The Effect of Er:YAG Laser Treatment on Biofilm Formation on Titanium and Zirconia Disc Surfaces
(Journal of Periodontology, 2023) Assery, Nasser; Tsigarida, Alexandra
Background: Lasers represent a promising method for implant decontamination, but evidence on implant surface changes and subsequent biofilm formation is limited. This study aimed to assess the effect of Er:YAG laser treatment on zirconia and titanium discs, and the differences in biofilm formation as a result of surface alterations. Methods: A two-stage (in vitro and in vivo) experiment utilizing Er:YAG laser on titanium and zirconia discs was performed. In vitro, surface alterations, roughness, and elemental-material weight differences following laser treatment were assessed using Scanning Electron Microscopy (SEM) and Atomic Force Microscopy (AFM). In vivo, four participants wore custom-made intra-oral stents, embedded with laser-treated and untreated titanium and zirconia discs overnight. Biofilm-coated discs were stained using nucleic acid fluorescence dye and visualized using multiphoton confocal laser scanning microscopy. Biofilm 3D structure, biomass, thickness, and live-to-dead bacteria ratio were assessed. Results: Both titanium (TiZir) and zirconia (YTZP) discs treated with Er:YAG laser resulted in visual surface alterations, but showed no significant change in average surface roughness (titanium P=0.53, zirconia P=0.34) or elemental-material-weight (TiZir, P=0.98), (YTZP, P=0.96). No significant differences on biofilm biomass, average thickness and live-to-dead bacteria ratio of laser-treated titanium and zirconia discs, were identified compared to untreated groups (titanium P > 0.05, zirconia P >0.05). Generally, zirconia discs presented with a lower live-to-dead bacteria ratio compared to titanium discs, regardless of laser treatment. Conclusion: Er:YAG laser treatment of titanium and zirconia implant surfaces does not significantly affect surface roughness, elemental–material weight, and early biofilm formation in the oral cavity.
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