Browsing by Author "Rasdan, Alia Saeed Mohammed"

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The Impact of Biologically Synthesized Nanoparticles on Colon Cancer Cells: In Vitro Cellular and Morphological Investigation
(2022) Rasdan, Alia Saeed Mohammed; خان، فردوس علم
Abstract: Colorectal cancer (CRC) attacks the colon or rectal region of the large intestine. CRC is the third globally prevalent cause of cancer mortality. Although current treatments are effective, they have several drawbacks such as systemic toxicity. Therefore, nanoparticles (NPs) are being investigated as delivery systems. Green synthesized NPs are eco-friendly, non-toxic, and safe to manufacture, making them a better option than chemically synthesized NPs. In this project, we tested green vs. chemically synthesized NPs with and without date extract to study their cytotoxic effects. The sol-gel approach was used to create CoFe2O4/(Ni0.4Cu0.2Zn0.4Fe1.8O4( NPs. Characterization was performed using XRD, SEM, TEM, and zeta potential to investigate the NP structure and morphology. These assays confirmed the chemical composition with size ranging from 24 to 41 nm. Furthermore, we performed MTT, DAPI, and cell migration on HCT116 (colorectal cancer) and HEK293 (human embryonic kidney) cell lines. Treatment groups included: (BS1, BS2, BS3, BS4, BS5, BS6, BS7, BS8, and S9) at varying concentrations for 48h. For HCT116, MTT results revealed that the lowest viability was 20.1% after treatment with BS8 and the highest was 23.76% in BS1. Whereas for HEK293, the lowest viability was 21.72% in S9 and the highest was 25.84% in BS6. The results obtained from DAPI assay suggest that all NPs inhibit the growth of both cell lines with significantly shrunken and fragmented nuclei. In cell migration, treatment with BS1, BS2, BS3, BS7, BS8, and S9 showed very few cells migrated across the empty areas in HCT116. Cell migration was observed in HEK293 following treatment with BS1, BS2, BS3, BS4, BS6, BS7, BS8, and S9 NPs. Thus, these results suggest that BS1, BS2, BS3, BS7, BS8, and S9 can be good candidates for CRC therapy. This research will help find a new therapy for CRC treatment.
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