Imam Abdulrahman Bin Faisal University
Permanent URI for this collectionhttps://drepo.sdl.edu.sa/handle/20.500.14154/16
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Item Restricted Studies on Cellulolytic Soil Fungi Isolated from Eastern Region of Saudi Arabia and their Application in Biofuel Production(Imam Abdulrahman Bin Faisal University, 2022) Almutairi, Asma Ayidh B; العبدالعال، اميرة حسن17 0Item Restricted Elastase production by a selected bacterial isolate from the Eastern Province of Saudi Arabia and its applications(Imam Abdulrahman Bin Faisal University, 2022) Mubarak, Ghadah Abdulaziz Alshaikh; Alabdalall, Amira13 0Item Restricted Biosynthesis of Silver Nanoparticles by Industrial Soil Isolate from the Eastern Province of Saudi Arabia(Imam Abdulrahman Bin Faisal University, 2021) Basalama, Huda Bobakr Saeed; الحمامي، ايناس محسن11 0Item Restricted Production of fluorescent siderophores by selected bacterial strains of Pseudomonas spp. isolated from the Eastern Province of Saudi Arabia(Imam Abdulrahman Bin Faisal University, 2021) Al Muhawish, Mashael Abdulraouf Abdullah; عبدالمنعم، عصام قطب; الخالدي، عيدة مرشد13 0Item Restricted Molecular Characterization and Antibiotic Resistance of UroPathogenic Escherichia coli (UPEC) Isolates from Patients within the Eastern Province of Saudi Arabia A(Imam Abdulrahman Bin Faisal University, 2018) Abusuliman, Mohammad Saleh; Elhadi, NasereldinAntibiotic resistance poses a global challenge in the control of bacterial disease and it is of serious concern in Saudi Arabia. Pseudomonas aeruginosa is one of the main causes of antibiotic resistance in Saudi Arabia and its production of metallo-beta-lactamases enables the pathogen to resist most antibiotics. This study assessed the antibiotic susceptibility and the occurrence of metallo-beta-lactamase genes such as blaVIM, blaNDM-1and INT-1 in 110 clinical samples obtained from King Fahd University Hospital. Pseudomonas aeruginosa strains were identified using VITEK 2 system. The identified strains were confirmed using Cetrimide agar after which antimicrobial susceptibility was identified using VITEK 2 antimicrobial susceptibility cards. Polymerase chain reaction based molecular screening techniques was used to analyze the presence of blaVIM, blaNDM-1, and INT-1. 108 strains of P. aeruginosa were identified. Most of the strains were identified from the urine (21.5 %) and wound samples (17.4 %). Most of the strains were susceptible to beta lactam antibiotics while the non-beta lactam antibiotics such as Tobramycin, Levofloxacin, Gentamicin, Amikacin, and Colistin were less susceptible. The ERIC-PCR finger printing showed that the strains have ERIC sequences of varying lengths as shown by variation in the migration distances. The dendogram showed twenty different ERIC clusters, with four major clusters. Most (65.7 %) of the identified strains had at least one of the three genotypes of interest with the highest number (48.1 %) being reported to be NDM producers while the VIM producers were the least frequent strains. The combination blaVIM and blaNDM genotypes were the most frequently observed gene combination among the identified strains of P. aeruginosa while the combination of blaVIM and INT1 occurred in the least number of strains. The information obtained from the study is important in strategizing on the best approach in the management antibiotic resistance caused by P. aeruginosa in Saudi Arabia.1 0Item Restricted Characterization of non-typhoidal Salmonella associated with gastroenteritis in Eastern Province of Saudi Arabia(Imam Abdulrahman Bin Faisal University, 2018) Al. Harbi, Adham Ghalib; Alomer, Amer; Elhadi, NasereldinBackground and Objective: Urinary tract infections (UTIs) are a common cause of serious public health problems, with UroPathogenic Escherichia coli (UPEC) being the predominant etiological agent in both community and healthcare settings. UPEC isolates exhibit a wide range of virulence factors (VFs), which enhance their virulence potential in establishing primary, recurring and chronic UTIs. Antibiotic resistance to UPEC is increasingly being reported, worldwide. The aim of this study was to undertake molecular characterization of the UPEC isolates and correlate the expression of specific virulence-associated factor genes (VFGs) with multi-drug antibiotic resistance from hospital settings in King Fahad Hospital of the University (KFHU) in AL-Khobar, an Eastern Province in the Kingdom of Saudi Arabia. Material and Methods: A total of 182 urine samples positive for UPEC isolates were collected from different patients between 1st October 2016 and 30th April 2017 and the identification confirmed with VITEK 2 system. All UPEC isolates were tested for extended spectrum beta-lactamase (ESBL)-production and antibiotic susceptibility against a total of 17 antibiotics using VITEK 2 system. Then all UPEC isolates were screened for VFGs using polymerase chain reaction (PCR) technique, where genotyping was performed using gene-specific primers against the different VFGs (fimH, papC, sfa/focDEh, hlyCA, Usp, and iucC). Plasmid DNA extraction was processed using centrifugation method. Statistical analysis of the data was performed using SPSS software. Results: Majority of UPEC isolates were from female (71.20%) than male patients with UTIs. On analysis of the VFGs, the majority of UPEC isolates (94.51%) expressed fimH gene followed by usp (65.38%), papC (58.24%), hlyCA (29.67%), sfa/focDEh (29.67%), and iucC (17.58%), with no statistically significant gender difference (p>0.05). About 28.18% of the UPEC isolates were ESBL-producers. The isolates were highly resistant against amoxicillin (66.67%), ampicillin (65.05%) and amoxicillin/clavulanic acid (45%); and highly sensitive to tigecycline, nitrofurantoin and sulfamethoxazole (0% resistant). Antibiotic resistance was significantly associated with SfafocDEh (47%), iucC (47%), usp (35.29%), HlyCA (29.41%), and papC (23.5%) genes (p<0.05), but not fimH. However, fimH gene was significantly associated with UPEC resistance to amoxicillin/clavulanic acid (p=0.018). Conclusion: Findings from this study indicate that all the six VFGs are important for the pathogenicity of UPEC isolates. The fimH gene, which is ubiquitous in both E. coli commensals and UPEC strains, is poorly associated with antibiotic resistance of UPEC isolates. All the VFGs except the fimH gene are associated with antibiotic resistance. ESBL-production is a strong predictor of antibiotic resistance in nearly all classes of antibiotics. These findings necessitate revamped surveillance programs for resistant UPEC isolates and a review of antibiotic prescribing practices in Saudi Arabia. These would put to an end, the random prescription policies involving the classes of the antibiotics evaluated in this study.2 0Item Restricted Prevalence Of Metallo Β-Lactamase Producing Pseudomonas Aeruginosa In Eastern Province Of Saudi Arabia A(Imam Abdulrahman Bin Faisal University, 2018) Al- Buloshi, Ahmed Fouad; Elhadi, NasereldinCerebral Palsy (CP) is a disorder that affects one’s posture and movement, and it can be related to sensory, cognitive, perceptual, and psychological disturbances (Sankar and Mundkur, 2005; Bax, Flodmark and Tydeman, 2007). This disorder's etiology is multifactorial in nature, and it is possible during the prenatal, perinatal, or post-natal periods (Sankar and Mundkur, 2005). Children suffering from CP have many neurological deficits, which interfere with their motor functions and, in turn, their ability to engage in daily activities (Gormley, 2001). Hence these activities are affected. Performing a functional assessment has become a necessary part of a rehabilitation program to measure the program's effectiveness and the patient's clinical progress (Hall et al., 1993). Therapeutic intervention with CP children should primarily strive toward equipping such children with the relevant skills to help them execute essential tasks and engage in day-to-day activities. These activities of daily living are associated with life, such as self-care, transfers, mobility, communication and social cognition (Tur et al., 2009). However, few studies have addressed the performance of daily activities in children with CP (Ostensjø, Carlberg and Vøllestad, 2003) Beyond the consideration of functional activities, physiotherapists must also keep participation in mind while assessing cases of patients suffering from CP. Participation is becoming increasingly significant in childhood, particularly in the realm of childhood disability, since children with disabilities are typically more constrained when participating in recreational activities, daily activities, social events and sports than their peers. This leads to low life satisfaction as children turn into adults, and this gap widens as they grow up. Importantly, it is not merely a child's physical or mental ability that is associated with participation. In contrast, participation engages with multiple other factors within the child and the environment wherein a child's growth occurs. For example, family perceptions, gender, age and family income are linked to participation in many previous studies (Anastasiadi and Tzetzis, 2013). Another aspect often undermined and frequently overlooked is the caregiver's quality of life. This factor plays a vital role in the life of children suffering from CP. Over the past two decades, healthcare strategies and technological advances in medicine have radically increased the life span of many children suffering from CP. Simultaneously, many parents have started seeking innovative ways to raise their children with CP at home and include them in a productive and meaningful manner in their communities. In addition, a home environment can 2 maximize the child's capabilities, minimize the adverse effects of the impairments and reduce or completely prevent the hospitalization stay period ('Guidelines for home care of infants, children, and adolescents with chronic disease. American Academy of Pediatrics Committee on Children with Disabilities,' 1995). Despite such positive benefits and the consideration that caregiving is a natural component of parenting young children in general, the demands associated with facilitating high levels of care required by a child with functional limitations that persist in the long term can quickly become burdensome for the caregivers and, in turn, affect their physical and mental health and well-being (Raina et al., 2004). Moreover, the families of children with disabilities have to handle more significant financial stress, struggle with frequent disruptions of family routines, and reduced social activities (Thyen, Kuhlthau and Perrin, 1999). Only a few studies have directly addressed how the mothers' quality of life is affected due to their engagement in caregiving activities for children with a disability (Eker and Tüzün, 2004). Hence Saudi Arabia has a different culture than the western world, and CP is a common disorder here; we are interested in finding the children's functional capacities, participation levels, along with their caregiver’s burden and various factors affecting these parameters along with the relationship between these variables.0 0Item Restricted Prevalence of CTX-M Beta-Lactamase Producing Escherichia coli in Eastern Province of Saudi Arabia(Imam Abdulrahman Bin Faisal University, 2018) Al-Harshan, Hazmah Ali; Samir, ElbarbaryEscherichia coli known for its ability to resist the beta lactam antibiotic group by the production of beta lactamases enzymes, which will hydrolyze broad spectrum of beta-lactam antibiotics and known as ESBL E. coli. Globally, there is more attention on the spread of ESBLs E. coli because the presence of this enzyme will lead to more difficulties in antibiotic selection. The three main ESBL genes are bla SHV, bla TEM and bla CTX-M. The CTX-M ESBL family recently became the most predominant type of ESBL worldwide, and is referred to as the "CTX-M pandemic". Clinical samples were obtained from patients at King Fahad Hospital in Alkhobar city in Eastern Province of Saudi Arabia. Identification and sensitivity was carried out by a VITEK2 analyzer. All ESBL E. coli producers were reconfirmed on an ESBL chromogenic agar. All strains have been through genomic DNA extraction, polymerase chain reaction with specific primers (U-CTX-M, CTX-M-1, CTX-M-9, CTX-M-14, and CTX-M-15), and then went through gel electrophoresis. Plasmid DNA extraction was conducted with a QIAprep Spin Miniprep Kit. A total of 220 clinical samples were obtained, and 30.9% (68/220) of the isolates were ESBL producers. Most of the samples were urine (184/220). ESBL-E. coli showed a high rate of resistance, including trimethoprim/sulfamethoxazole 77.9%, ciprofloxacin 67.7% and gentamicin 26.5 %. Imipenem and Meropenem were both recognized as resistant in 1.5% (n=1) of the isolates. CTX-M was found in 88.2% (n=60) of ESBL E. coli. The distribution was as follows: CTX-M-1 95 %, CTX-M-9 10%, CTX-M-14 21.7% and CTX-M-15 55%. Plasmid was obtained from 53.3 % of all the CTX-M positive isolates. In conclusion, ESBL was found to be high among isolates, most of them harboring the CTX-M gene with the most prevalent CTX-M-1 type. Antibiotic resistance was high among ESBL isolates, and carbapenems was the best treatment choice, hence the recent emergence of carbapenemase is a great concern4 0Item Restricted Prevalence and Molecular Characterization of Extended Spectrum Beta- Lactamases Among Escherichia coli and Klebsiella pneumoniae Isolates from Clinical Specimen in Eastern Province of Saudi Arabia(Imam Abdulrahman Bin Faisal University, 2017) Al-Boqami, Hanan Ahme; Elhadi, Nasreldin6 0