Saudi Cultural Missions Theses & Dissertations
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Item Restricted Part 1: Studies on the Microbial Community profile of Two Caves in Greece and Slovakia. Part 2: Studies on the effect of Geographical and environmental factors on tea metabolites profile.(Saudi Digital Library, 2023-11-06) Almutari, Saleh; Kumaresan, Deepak; Di, WuThis comprehensive study delves into two distinct, yet interconnected realms of scientific inquiry: the microbiology of cave ecosystems and the metabolomics of tea. The first part of the study focuses on the microbial diversity in cave ecosystems, specifically in Crete (Greece) and Slovakia. Utilizing advanced techniques such as Oxford Nanopore Technologies for DNA sequencing and MicrobiomeAnalyst for data analysis, the research uncovers the complex microbial communities within these caves. Key findings include the dominance of certain microbial families like Sphingomonadaceae and the variation in microbial diversity between different cave locations. The study highlights the significant role of these microorganisms in cave ecosystems and sheds light on their potential biotechnological applications. The second part of the research investigates the impact of environmental and geographic factors on the metabolome profile of tea. Employing methods such as Liquid Chromatography-Mass Spectrometry (LC-MS) and Geographical Information Systems (GIS), the study examines how factors like altitude, soil composition, and climate affect the metabolite composition of tea. The results reveal distinct metabolomic fingerprints for teas from different geographic locations, underscoring the influence of environmental conditions on tea's flavor, aroma, and health benefits. The study contributes valuable insights into the metabolomics of tea and its implications for the tea industry, conservation, and health research. Overall, this dual-focused study bridges the gap between microbial ecology and plant metabolomics, offering a holistic view of the biological diversity in both subterranean and agricultural environments.14 0