Saudi Cultural Missions Theses & Dissertations

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    Insect-Based Protein: Nutritional Analysis and Their Role in Sustainable Development
    (University of Birmingham, 2024) Albarakate, Mohammed; Miri, Taghi
    This study investigates insect-based proteins as a sustainable alternative to traditional animal sources. Consider their nutritional content, bioactive compounds, sensory qualities, potential risks, and environmental impact. Utilizing a bibliometric analysis of research from the Scopus database, the study observed a considerable increase in scholarly interest beginning in 2013, driven by the publication of the Food and Agriculture Organization's report "Edible Insects: Future Perspectives of Food and Nutrition Security." In 2015, the European Union adopted insects as a novel food, contributing to ongoing research. This increase is especially noticeable in Western countries, where eating insects is uncommon. The study emphasizes insects' high nutritional value, which includes energy, protein, essential amino acids, healthy fats, dietary fiber, vitamins, and minerals. They also include bioactive compounds such as antioxidant and anti-inflammatory properties, which make them beneficial to human diets. The study also highlights the importance of consumer innovative product development, and further research on novel processing techniques to enhance the sensory qualities of insect-based protein. It also investigates the potential health hazards associated with their ingestion, including microbiological hazards chemical contamination, allergenicity, and antinutrients, emphasizing the significance of proper safety measures. Insect farming is an environmentally friendly alternative that can considerably reduce greenhouse gas emissions, land and water use. Utilizing food waste as feed enhances sustainability. Insects have a high feed conversion ratio, making them a very effective source of protein. Therefore, incorporating insects into diets can have a positive impact on environmental sustainability, food security, and overall health of current and future generations.
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    Solid-Phase Synthesis of s-Tetrazines: Method Development and Applications in Chemical Biology
    (The University of Edinburgh, 2024) Alghamdi, Zainab Saeed A; Lilienkampf, Annamaria; Brechin, Euan; Bradley, Mark
    Since the emergence of bioorthogonal chemistry in the 20th century, s-tetrazines stand out from the bioorthogonal toolbox due to their superior reaction kinetics over azide/alkyne chemistries, and high chemical selectivities. Specifically, s-tetrazines selectively undergo inverse electron-demand Diels-Alder reactions with dienophiles within complex biological systems, which has allowed for their use in various biological scenarios such as in sensing, imaging and drug delivery. However, despite the growing applications of tetrazine bioorthogonal chemistry, their development has been hampered due to a lack of practical approaches that facilitate the emergence of new applications. In this thesis, an efficient and high-yielding solid-phase route to s-tetrazines was successfully developed without the need for metal catalysts or harsh reaction conditions. This method provides a versatile route to the synthesis, under mild conditions, of unsymmetrical aryl and alkyl tetrazines, as well as more reactive monosubstituted tetrazines, with possible applications in bioorthogonal chemistry. To explore the bioorthogonal activation of tetrazines and of mycobacteria labelling, a small library of fluorescent probes was initially developed based on the mycobacterial membrane-associated disruption peptide (MAD1), with evaluation of both enantiomers of the MAD1 peptides, decorated with four different fluorophores (ranging from green to far-red), including solvato-fluorogenic dyes. Fluorescent labelling on M. smegmatis, showed robust and stable labelling. Two of the D-probes (5-CF-D-MAD1 and NBD-D-MAD1) successfully labelled Mtb. A norbornene-labelled MAD1 peptide was then synthesised, which was used to bind M. smegmatis, and trigger the activation of the quenched fluorophore (BODIPY-Tz). This process facilitated a 61-fold amplification of the fluorescent signal upon bioorthogonal activation, allowing efficient free-wash labelling.
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    The impact of the Hilotherapy mask on the quality of postoperative recovery following orthognathic surgery: A prospective study
    (University of Glasgow, 2024) Banjar, Assim; Benington, Philip; Ayoub, Ashraf
    The Hilotherapy cooling and compression system consists of a mask made of polyurethane thermoplastic material, contoured to follow the shape of the face to regulate and maintain the temperature of the skin and maxillofacial cutaneous tissues via the mask water channel. Watercooled face masks have been used in orthognathic patients, with the aim of improving patient recovery and reducing postoperative swelling. Our study aims to assess the effectiveness of a Hilotherapy mask in reducing pain and improving patients’ recovery and QoL following orthognathic surgery via a questionnaire evaluating patients’ health statement and their satisfaction with the Hilotherm cooling mask after orthognathic surgery. Our results showed an overall satisfaction and improvement in patients’ health wellbeing QoL, self-care, mobility restrictions, and mental health (depression and anxiety) during the treatment. These findings indicate a positive outcome for these patients. It also suggests a protocol to improve patient recovery after orthognathic surgery with a limited use of steroids.
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    Navigating National Transformation: The role of project managers in strategy implementation within the context of Saudi Vision 2030
    (University Collage London, 2024-01) Ababtain, Jori; Vernet, Antoine
    Project management is critical in attaining an organization’s goals and objectives. Its significant role requires project managers to implement processes that align with the organization’s business strategy. However, it is common for projects to fail due to misalignment between project management and strategy management. This study was conducted to examine the crucial role of project management in progressive and ambitious business practices, especially in the case of giga-projects. The research outlined Saudi Arabia's Vision 2030 giga-project, exploring the importance of project managers and alignment between Project Management Offices (PMOs) and Strategy Management Offices (SMOs) for a successful project.
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    Exploring Gender Differences in Saudi EFL Learners' Attitudes and Comprehensibility Towards American and British English.
    (University of Reading, 2024-09-13) Asiri, Shamah; Setter, Jane
    Abstract This study was an attempt to explore how Saudi EFL learners feel about American and British accents and how well they understand these accents, with a focus on exploring differences between male and female learners. A number of 84 participants, both males and females, took part in the research, allowing for a balanced look at gender perspectives. Using a combination of questionnaires to assess attitudes and a verbal guise technique to assess how understandable these accents are, the study sheds light on how gender can shape accent preferences and comprehension. The insights gained from this research are valuable for educators and linguists who work in diverse, and in educational settings.
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    Intracellular and extracellular immunisation for HIV
    (The University of Manchester, 2024-11) Alatiq, Abdulrahman; Klapper, Paul; Valley, Pamela; Faqih, Layla
    The prevalence of HIV infection remains a serious challenge for public health. HIV infection results in considerable mortality and morbidity, as well as imposing a substantial financial burden on healthcare systems globally. Despite the availability of effective antiretroviral treatments to control infection, an effective protective vaccine remains elusive. This project aimed to develop a practical, affordable, and effective combined vaccine and treatment by combining intracellular antibodies and extracellular immunisation to prevent or reduce HIV viraemia. Specifically, an approach in which DNA-encoding N49P7 broadly neutralising antibodies against the HIV-1 envelope glycoprotein 120 is delivered to cells using recombinant baculovirus or lipid nanoparticle (LNP) transfection. Extracellular antibodies were anticipated to prevent gp120 from attaching to cells, and intracellular antibodies were intended to inhibit the genesis of virions. The expression of N49P7, either as a human IgG antibody or as human Fab, was attempted. The expression of the N49P7 IgG antibody was not achieved in HEK 293 cells despite employing three delivery models for transfections: recombinant baculovirus, MC3-based LNP, or lipofectamine 2000 LNP. While transfection was successfully achieved (monitored via an eGFP gene included within the plasmid design), functional antibody was not achieved. The most likely explanation for the failure was thought to be the use of dual promoters within the expression cassette. Redesign of the plasmid to create a bicistronic vector including the N49P7 Fab region and signal peptide sequences of murine IgG and IL-2 allowed successful expression of N49P7 Fab through recombinant baculovirus or lipofectamine 2000 reagent transfection of HEK 293 cells. However, the expressed N49P7 Fab region was predominantly accumulated intracellularly. A further redesign was implemented to incorporate homogenous signal peptides H7 and L1 into the N49P7 Fab gene, which significantly enhanced the secretion of the antibody fragment. This design maintained functional intracellular and extracellular antibody activity. Lower cellular cytotoxicity was seen with recombinant baculovirus transfection compared to lipofectamine 2000 LNP mediated transfection although both were equally efficient. The selection of an optimum method for transfection will form part of future investigations progressing to animal model testing. This proof of principle study showed that recombinant baculovirus or lipofectamine 2000-mediated transfection systems allowed efficient N49P7 Fab expression both intracellularly and extracellularly in mammalian cells, suggesting that this approach indicates potential for providing a vaccine against HIV infection in addition to a therapeutic intervention for those who already have HIV infection.
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    Factors Influencing Critical Care Nurses’ Attitudes to Patient Safety: A Systematic Review
    (QUEEN’S UNIVERSITY, BELFAST, 2024-09-08) Alduways, Saleh; Claire, Kerr
    Background: Ensuring patient safety continues to be a key aspect in the health sector. Negligent practices concerning health, hygiene and diet are among the top killers in modern society. The attitudes and practices of critical care nurses concerning patient safety culture are of significant importance. Safety culture refers to the safety beliefs, values and attitudes adopted by health care providers, as well as the processes of practising them and the dedication to creating a riskless health care system. Aim: This systematic review seeks to summarise the attitudes and practices of critical care nurses towards patient safety in ICUs and their adherence to international safety goals, and factors that affect their attitudes and practices towards patient safety practices. Methods: A systematic literature search was conducted in four online databases – CINAHL, MEDLINE, PubMed and Scopus – of publications spanning September 2014 to 2024. The PICo framework was employed in the formulation of the research question. Specific criteria were set for the inclusion of studies. The quality of the included studies was assessed using the Appraisal tool for Cross-Sectional Studies (AXIS). Results: Ten studies were included in this systematic review. These studies employed cross-sectional design to investigate critical care nurses’ attitudes, perceptions and practices about patient safety, compliance with international safety goals, and factors that may influence them in critical care settings. In general, critical care nurses reported significant negativity in safety culture, and the management perception scores were low overall. Organisational commitment and job satisfaction had the highest means of positive scores as did teamwork. Nurses likewise confirmed a perspective of International Patients Safety Goals (IPSGs) as a reliable framework for patient safety; individual factors affecting patient safety culture included fatigue, workload and emotional exhaustion. Nurses aged 31–35 were more knowledgeable about the IPSGs. Conclusion: The current study reveals that critical care nurses had negative perception towards safety culture and poor response on the management support and the implementation of some principles of patient safety. Although 8 nurses’ knowledge of specific goals is strong, a number of implementation challenges remain and thus require focused interventions to enhance safety culture.
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    Investigation of the feasibility and utility of electronic patient-reported outcome measures in Paediatric Dentistry clinical practice
    (University of Sheffield, 2024-06) Attamimi, Sultan; Gilchrist, Fiona; Marshman, Zoe; Deery, Chris
    Background: There is a shift within healthcare services towards incorporating Patient-Reported Outcome Measures (PROMs) rather than relying solely on clinical outcomes. There is a lack of evidence exploring the routine clinical use of PROMs in children’s oral health. Aim: To explore the feasibility and utility of web-based electronic PROMs in routine clinical practice in paediatric dentistry. Design: The first stage involved the development of the electronic Personal Assessment Questionnaire-Paediatric (ePAQ-PD) using paediatric oral health PROMs, Caries Impacts and Experiences Questionnaire for Children (CARIES-QC) and Children’s Experiences of Dental Anxiety Measure-short form (CEDAM-8) and additional free-text questions. Next, technical and usability testing was conducted to ensure readiness of the ePAQ-PD for routine use. This was followed by implementation of the ePAQ-PD in routine practice with a purposive sample of children, parents/carers and clinical staff interviewed to explore their views on the feasibility and utility of the ePAQ-PD. Finally, the psychometric properties of the eCARIES-QC were evaluated using classical test theory and Rasch model analysis. Results: The ePAQ-PD showed acceptable technical and usability performance. Children (n=237) and their parents/carers with varying socio-demographic characteristics completed the ePAQ-PD with a response rate of 69.5%. A range of views were captured in terms of children’s preferences, acceptability of the electronic format, role in clinical effectiveness and potential impact on treatment planning. The ‘Question to Dentist’ item was found to be effective in capturing information and concerns from children and parents/carers. Areas for improvement were suggested including the content of the invitation text, intuitiveness and child-friendliness of the design. Clinical staff requested improvements in accessibility and readability to facilitate faster interpretation. The eCARIES-QC showed strong construct validity (p < 0.001) and a high Cronbach's alpha of 0.91 with a good fit to the Rasch model. The free-text question captured additional impacts mainly related to pain, sensitivity, aesthetics and anxiety. Conclusion: The ePAQ-PD had acceptable feasibility and utility for routine use in paediatric dental practice. The ePAQ-PD has been shown to improve children’s involvement in their oral care, their clinical experience and communication with clinical staff. eCARIES-QC had acceptable psychometric properties for a wider age range than initially designed and appeared to capture the impacts of non-caries-related oral conditions with high reliability. The free-text questions captured further valuable impacts that should be used to complement the eCARIES-QC in routine clinical practice.
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    In-Vitro Effects of Recombinant IL-37 protein on Bladder cancer cell line Growth and Proliferation
    (University of East London, 2024-08) Alamri, Ashwaq; Gobena, Edessa
    Introduction: Interleukin-37 (IL-37) is a cytokine of the IL-1 family, recognized for its anti-inflammatory and immunomodulatory functions. Despite growing interest in IL-37’s role in different types of cancers, its impact on bladder cancer growth and proliferation has not been thoroughly investigated. This study aims to explore the effects of recombinant IL-37 protein on the viability, proliferation, and apoptosis of T24 bladder cancer cell lines, addressing a significant gap in current cancer research. Methods: In this in vitro study, T24 bladder cancer cells were treated with different concentrations of recombinant IL-37 protein (1 μg/ml, 0.5 μg/ml, and 0.25 μg/ml) with negative control (0 μg/ml). Cell viability and apoptosis were assessed using flow cytometry, and the levels of pro- inflammatory cytokines IL-6 and IL-8 in the cell culture supernatants were quantified using enzyme-linked immunosorbent assay (ELISA). Statistical analysis was performed using one-way ANOVA followed by Tukey’s post hoc test to determine the significance of the results. Results: The results showed that IL-37 induces apoptosis in a dose-dependent manner, with the highest concentration (1 μg/ml) significantly reducing the percentage of viable cells from 90% to 71% and increasing the apoptotic cell population from 7% to 27%. Additionally, IL-37 treatment modulated the secretion of IL-6 and IL-8, with a significant increase in IL-6 production at 0.25 μg/ml (P ≤ 0.05) compared to the control. However, IL-37 did not significantly inhibit IL-8 production across the different treatment groups. (P ≤ 0.05). Conclusion: This study demonstrates that IL-37 not only promotes apoptosis in bladder cancer cells but also alters the tumor microenvironment by modulating cytokine secretion. These findings suggest that IL-37 may hold therapeutic potential as a novel approach to bladder cancer treatment. Further research is necessary to validate these results in vivo and to elucidate the precise molecular mechanisms through which IL-37 exerts its anti-tumor effects.
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    Hospital-Based Nurses' Experiences of Caring for Dementia Patients: A Systematic Review
    (Queen's University Belfast, 2024-10) Musawi, Abdullah; Brown, Michael; Galway, Karen
    Background As the prevalence of dementia rises globally, with notable increases being seen in regions such as Saudi Arabia due to an ageing population, it is essential to understand patients’ experiences to effectively address negative incidents that hinder quality care and improve nursing education, healthcare organisation environments, and nursing practice. Objectives The objectives of this study are to (1) synthesise qualitative evidence regarding hospital staff experiences in caring for PLWD, (2) identify barriers and facilitators in providing person-centred dementia care, and (3) explore the impacts of this care on nurses, including challenges such as workload and stress, as well as positive aspects like fulfilment and satisfaction. Methodology A comprehensive search was conducted across credible databases, such as PubMed and EBSCO, focusing on studies published from 2019 to 2022, and guided by inclusion and exclusion criteria to ensure relevance and quality. Study quality was assessed using the CASP checklist, and findings were synthesised using meta-aggregation, with similar findings being grouped into categories to allow for a structured narrative synthesis. Findings Identified themes from the ten selected studies were as follows: 1) emotional impact and satisfaction, 2) challenges in providing person-centred care, 3) the importance of specialised knowledge and skills, 4) collaboration and teamwork, and 5) adaptability in medication management. Experiences encompassed a range of emotions, including frustration and fulfilment, communication issues, lack of training, and environmental barriers. Limitations included a small sample size of only ten studies and the exclusion of non-English and quantitative research. This potentially skewed the representativeness and depth of the findings while overlooking variations in experiences influenced by nurses' backgrounds or specific training. Conclusion These findings underscore the need for targeted interventions that support nurses' professional development and emotional health in dementia care settings.
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