Saudi Cultural Missions Theses & Dissertations
Permanent URI for this communityhttps://drepo.sdl.edu.sa/handle/20.500.14154/10
Browse
2 results
Search Results
Item Restricted IN VITRO COMP TRO COMPARISON OF CELL ARISON OF CELLULAR CYTOXICITY OF CONVENTIONAL VS. CAD/CAM DEN AL VS. CAD/CAM DENTURE BASE M TURE BASE MATERIALS(Saudi Digital Library, 2024) albasry, zainab; Ayman, Ahmed; Daniela, Masson Mayers; Jack, KeeslerAim: Adverse reactions and local irritation of the oral mucosa to prostheses constructed from PMMA include allergic response, erythema, and a burning sensation of the tongue and mucosal tissues. Evidence on the cytotoxic effects of digitally manufactured denture base materials remains scarce. The aim of this study was to evaluate and compare the cytotoxic effects of CAD/CAM fabricated denture bases versus conventionally heat-cured acrylic resin materials. Materials and Methods: In total, 75 disks of five different denture resin materials – 15 disks per group were prepared: Ivocap (group 1; control), Ivotion denture base (group 2), Lucitone 3D denture base (group 3), Sprintray (group 4), and Flexera (group 5). Eluates of the materials from 1- and 7-days incubation in cell culture medium were used on human gingival fibroblasts seeded in 96-well microplates. Cytotoxicity was evaluated by MTT assay for both time points, and percentage cell viability was estimated. Effects on fibroblasts were also examined by Live/Dead fluorescence assay and phase contrast microscopy. MTT assay results were analyzed using analysis of variance (ANOVA) followed by Tukey’s post-hoc test. paired sample t-test was used for day-wise comparisons. Results are presented as mean±SD and p-values less than 0.05 were considered statistically significant. Results: MTT assay showed a lower cell viability for groups one and five when compared to the other groups. Statistically significant differences were detected between groups at day 1 [group 1 x 3 (p=0.0055), 1 x 4 (p=0.0025), 2 x 5 (p=0.0004), 3 x 5 (p<0.0001) and 4 x 5 (p<0.0001). No statistically significant differences between the groups were noted at day 7 MTT assay. Paired t-test indicated a decrease in cell viability at day 7 for groups 1 to 4 (p=0.0398; p=0.0075; p=0.0345; p<0.0001, respectively), indicating that exposure time affected viability. Microscopic analyses correlated with MTT findings and showed morphological changes for group 5 at day 1 incubation with extracts and group 1 at day 7 incubation. Conclusion: Cell viability levels were above the average threshold of ISO cytotoxicity potential standards, indicating the suitability of the studied denture base materials in clinical prosthodontics. However, all materials showed some degree of reduction of cellular viability.6 0Item Restricted In Vitro Cytotoxic and Genotoxic Assessment of Nano Polyethylene Terephthalate Exposure on A549 Cells(University of Copenhagen, 2023-05-30) Alzaben, Mohammad; Roursgaard, MartinGlobal plastic production in 2019 amounted to 359 million tons, with the European Union contributing 17%. Plastic has caused environmental and health concerns. Nanoplastics, tiny fragments of plastic measuring less than 1μm in size, have become ubiquitous in the environment. As a result of plastic pollution and poor waste management, nanoplastics have infiltrated several ecosystems, including oceans, freshwater bodies, and even the air we breathe. Studies have linked exposure to MP/NP to adverse health effects such as Pneumoconiosis, chronic bronchitis, allergic and asthmatic reactions, and lung and digestive system cancer, raising concerns about their potential health effects. This study aims to assess two main biological adverse effects of nanoplastics, cytotoxicity and genotoxicity, on human lung carcinoma epithelial cells (A549). We chose polyethylene terephthalate (PET) because it is one of the top-produced plastic polymers. We found that NPET significantly induces ROS production and DNA strand breaks = 0.10 lesions/106 bp at the highest concentration (125 μg/ml).10 0