GROWTH EFFECTS OF TUMORIGENIC CELLS EXPOSED TO EXTRACTS OF THE PLANT BOSWELLIA SACRA

Abstract

Growth Effects of Tumorigenic Cells Exposed to Extracts of the Plant Boswellia Sacra (Under the direction of DR. ELBERT LEWIS MYLES) The Boswellia sacra plant has been used as a medicinal component for many centuries to treat multiple illnesses. The existing research findings demonstrate that the methanolic extract of the plant inhibits cancerous cell growth along cell lines. Due to the potential of the Boswellia sacra plant species to treat many cancers, this experiment sought to study its inhibitory effects on lung (A549), breast (BT549), and prostate (DU145) cancer cell lines. The other aims of this research were to investigate anticancer activity and the way different concentrations of the extract affect the human lung, breast, and prostate cancer cell lines. The cell viability of Boswellia sacra methanolic extract on A549, BT549, and DU145 cancer cell lines was measured by Trypan blue exclusion cell viability assay. For all cell lines, cells were cultured in vitro in varying concentrations of methanolic acid obtained by serial dilution. The 96 well plates were incubated in an area containing 5 % carbon dioxide for a duration of 24 hours. The results were then read off a fluorescent spectrometer and expressed as mean standard deviation values. Paired t-tests were conducted to determine cell viability. The methanolic extract demonstrated anticancer activity in a dose dependent manner for all viable cells. For the breast and prostate cancer cell lines, the pattern was evident; while for the lung cancer cell line, thevi pattern was indefinite particularly because of a lack of cell viability at higher doses. Thus, increasing the dosage showed increased cancer growth inhibition, which signals that methanolic extract, is a promising drug for cancer treatment. To conclude, Boswellia sacra extracts can be used to treat lung, breast, and prostate cancers. However, in vivo and clinical studies are required to determine its efficacy.