SACM - Jordan
Permanent URI for this collectionhttps://drepo.sdl.edu.sa/handle/20.500.14154/9658
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Item Restricted Correlation of the Effect of a Different Ecological Location on Antidiabetic Activity for Moringa oleifera Arial Parts Extracts Growing in Saudi Arabia(Saudi Digital Library, 2025) Almuayqil, Ahmed Naif; Jaber, Saif Aldeen MohammadBackground: Diabetes mellites (DM) is a widely spread disease in middle east countries especially in Saudi Arabia due to the lifestyle, type of food, and the lack of physical activity. DM is associated with high risk to human life due to the macro complications and micro complications that have a major effect on cardiac, renal, and neuronal systems. Unfortunately, the synthetic medication used for the controlling of DM has serious side effects on human body which push the scientific committees to suggest a different method for controlling DM. Moringa oleifera is on of the plants used for controlling diabetes for its protectory activity, and blood glucose lowering effect. Thus, the cultivation of moringa and the ecological factors have a major contribution on its activity. Methodology: moringa plant leaves were collected from two different ecological places (Asir and Al-Jouf) followed by drying. The dried leaves were soaked with different polarity solvents systems (n-hexane, chloroform, methanol, boiled and microwaved water). Phytochemical screening was applied for moringa extracts for the detection of tannins, saponins, alkaloids, and glycosides. In addition, the presence of phenolic compounds was tested using 2,2-diphenyl-1-picrylhydrazyl (DPPH) antioxidant activity. plant extracts antidiabetic activity were tested in vitro through measuring its inhibitory activity against α-amylase and -glucosidase. Finally, exact mass spectroscopy was used for the active extracts and compared with dictionary of natural products database (DNP) for detecting the major compounds found in the active extracts. Results: methanolic, and different water extracts were found to have different chemical classes that can pose antidiabetic activity. Methanolic and boiled water extracts from both locations were found to exert a strong antioxidant activity compared to quercetin and ascorbic acid positive controls. Furthermore, methanolic and boiled water extracts were found to have a stronger inhibitory activity against α-amylase and -glucosidase when compared to acarbose positive control. All of the major compounds found in both extracts were reported before to have a strong activity in insulin sensitivity enhancement, inhibitory activity against α-amylase and -glucosidase, and protectory activity in scavenging oxygen reactive species (ORS). Conclusion: Two factors were majorly affecting the antidiabetic activity and antioxidant activity of moringa constituents’ rainfall and temperature. It was found that rainfall levels has a higher contribution in the concentration of the active major compounds which was translated to a better antioxidant and antidiabetic activity Keywords: Anti-diabetic, Moringa, Extract, α-amylase, health outcomes, Secondary Compounds, Antioxidant1 0