Imam Abdulrahman Bin Faisal University
Permanent URI for this collectionhttps://drepo.sdl.edu.sa/handle/20.500.14154/16
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Item Restricted Long Term Effect of COVID-19 on Pulmonary Functions, Physical Activities, Functional Capacities, and Quality of Life. A Cross-Section Study(Imam Abdulrahman Bin Faisal University, 2023) Alshammari, Maha Ata Hmoud; شنب، السيد عبد الحميدBackground: Acinetobacter baumannii, a bacterial species which demonstrates an elevated wide range, multidrug resistance to commonly prescribed antibiotics, has been linked to recent major global outbreaks, raising a major clinical concern. Its reduced antibiotic susceptibility is closely related to the acquisition of a potent carbapenemase, and/or intrinsic gene ‘’over expression’’ through insertion sequences. Hence, this study aimed at investigating the antimicrobial susceptibility and molecular mechanisms underlying β-lactam resistance in A. baumannii, isolated at an academic medical center.Methods: To understand the basis of resistance, 103 multi-drug resistant (MDR) A. baumannii isolates were collected, their antibiotic susceptibility was tested phenotypically (Vitek II), and then molecular analyses was performed, by testing a range of commonly encountered carbapenemases—OXA-51, OXA-23, NDM, VIM, and KPC.Results: All strains demonstrated pan-resistance to most of the advanced antibiotics tested, including piperacillin/tazobactam, ceftazidime, cefepime, and ciprofloxacin. Moreover, majority of isolates exhibited resistance to imipenem (98.1%) and trimethoprim (90.3%). Approximately 50% of the strains showed meropenem, amikacin, and gentamycin resistance; however, lower resistance rate to tigecycline (4.9%) was noted. Moreover, isolates contained potent carbapenemases such as the intrinsic OXA-51 (89.3%), as well as the acquired resistant genes OXA-23 (68.9%), NDM (84.5%), and VIM (88.3%). The insertion sequence element ISAba1 was only detected in 35.9% of the strains.Conclusion: Potent resistant genes known to be carried on mobile genetic elements that aid the spread of highly resistant phenotypes were observed in a majority of isolates. These findings enforce the need for vigilant infection control measures and continuous surveillance.5 0Item Restricted Covid-19 Perceptions and Attitudes Towards Vaccination among Vulnerable Saudis: A Qualitative Study(Imam Abdulrahman Bin Faisal University, 2022) Al Beajan, Hebah Mahmoud; Alshawan, Deema10 0Item Restricted Menstrual Cycle Disturbance and its Risk Factors among Adult Females at King Saud University: Special Emphasis on COVID-19 Vaccines’ Effects(Imam Abdulrahman Bin Faisal University, 2022) Alghamdi, Taghread Saadi; عمر، التجاني عثمان2 0Item Restricted Impact of E-Recruitment on Business Performance : The Moderating Role of Pandemic or Covid-19(Imam Abdulrahman Bin Faisal University, 2021) Al-Mansour, Ali Jufaysh Saeed; Khan, Maqsood Mahmood27 0Item Restricted The psychosocial experience of healthcare providers during COVID-19 pandemic crisis in Saudi Arabia(Imam Abdulrahman Bin Faisal University, 2021) Al-Motawa, Tahany Essa Ali; Althumairi, Arwa14 0Item Restricted The Impact of Coronavirus Disease 2019 (COVID-19) on the Survivorship of Leukemia Patients: A Retrospective Cohort Study(Imam Abdulrahman Bin Faisal University, 2021) Almulhim, Fatima Abdulaziz Abdulrahman; Bah, Sulaiman9 0Item Restricted Parents’ Perception of the Scheduled and Proposed Children’s Vaccine against Covid-19 in Qatif, Eastern Province of Saudi Arabia(Imam Abdulrahman Bin Faisal University, 2021) Al Ghareeb, Fatimah Abdullah Ali; القحطاني، منى1 0Item Restricted Efficacy and Safety of Tocilizumab in COVID-19 patients: A Retrospective Observational Study(Imam Abdulrahman Bin Faisal University, 2021) Al-Qaaneh, Ayman Mohï؟½ï؟½ï؟½D Ramadan M; بيرجيو، فرانسيس7 0Item Restricted Development of a Colorimetric SARS-CoV-2 Detection Technology Using Reverse-Transcription Loop-mediated Isothermal Amplification (RT-LAMP) Assay(2022) Alhamid, Galyah Mohammed Abdullah; اوغلو، حسين كومبولThe ongoing COVID-19 remains to spread worldwide, causing new cases and deaths daily since its declaration as a pandemic over two years ago. Diagnostic tests are the first line of defense against the transmission of this disease, with the reverse-transcription quantitative polymerase chain reaction (RT-qPCR) being the approved gold standard. However, due to the global demand for facilities, reagents, and healthcare workers needed for rapid population-based testing, alternative tests need to be invested. The most common limitation in using RT-qPCR is giving false diagnoses, which puts others at risk of infection. Efficient fighting against this pandemic correlates with easy and large-scale accessibility of detection tools. Therefore, we developed a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay to diagnose COVID-19 by targeting and amplifying the SARS-CoV-2 E gene. The reaction takes place isothermally in a water bath set at 65 °C, and the positive results are read out with the naked eye via a color change from red to yellow, without requiring expensive and bulky instruments. In addition, a fluorometric RT-LAMP assay was developed to validate the results. Both colorimetric and fluorometric assays performed superbly when testing 150 clinical specimens, yielding 89.5% and 100% sensitivities, respectively. Optimizing the originally designed primer set (E-ID1) successfully detected SARS-CoV-2 in 20 minutes with a limit of detection of 500 copies/reaction, corresponding to 20 copies/µL, with no cross-reactivity observed among other respiratory viruses. No misamplification was evident after prolonged reaction times, which eliminates the most common limitation of potential false-positive diagnoses. Furthermore, we used a color sensor to demonstrate that the RGB codes gradually increase with the color change in positive RT-LAMP reactions, providing a simple alternative monitoring technique. Lyophilization of the colorimetric RT-LAMP reagents showed promising results to develop in-home and easy-to-store testing kit. These findings are essential to support the use of this technique in healthcare systems in fighting the COVID-19 pandemic, especially in resource-limited areas.11 0