Imam Abdulrahman Bin Faisal University
Permanent URI for this collectionhttps://drepo.sdl.edu.sa/handle/20.500.14154/16
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Item Restricted Length of Stay in Emergency Departments in Eastern Province, Saudi Arabia: Its Determinants and Effect on Patient Outcomes(Imam Abdulrahman Bin Faisal University, 2022) Alqattan, Khaled; المحلى، عزة علي3 0Item Restricted The Impact of Coronavirus Disease 2019 (COVID-19) on the Survivorship of Leukemia Patients: A Retrospective Cohort Study(Imam Abdulrahman Bin Faisal University, 2021) Almulhim, Fatima Abdulaziz Abdulrahman; Bah, Sulaiman5 0Item Unknown Associations between Tobacco Smoking, Mental Health and Health-Related Quality of Life Among Male Secondary Industrial Institute Students in Dammam, Saudi Arabia(Imam Abdulrahman Bin Faisal University, 2021) Al Kalif, Mohammed Sheker H; الغامدي، آمال على2 0Item Unknown Phenotypic and Genotypic Characterization of von Willebrand Factor Gene of Saudi Healthy Individuals in Eastern Province of Saudi Arabia(Imam Abdulrahman Bin Faisal University, 2020) Aldossary, Nemat Abdullah Ahmed; Gameel, Fathelrahman Mahdi HassanBackground:Vibrio parahaemolyticus is a gram-negative, facultative, anaerobic,halophilic bacterium that is widely disseminated in coastal, estuarine, andmarine environments. It is a seafood-borne pathogen that, in humans, cancause gastroenteritis. There are many clinical symptoms, such as diarrhea,abdominal pain, fever, and vomiting. Not all strains of V. parahaemolyticusare pathogenic, except, those are expressed tdh or trh genes. The polymerasechain reaction is the most popular molecular technique used to detect V.parahaemolyticus virulence genes. Recently, many studies have used anewly established technique known as immunomagnetic beads (IMB) toconcentrate the bacterium. This will increase the possibility of detecting V.parahaemolyticus from environmental, food, and clinical samples. Thisstudy aims to use PCR and IMB to detect V. parahaemolyticus in coastalwater samples.Method and result:From February 2018 to May 2018, seawater samples were collected fromfive different areas along the Arabian Gulf Coast in Saudi Arabia, includingAziziya Beach (AZB), Corniche alkhobar (KBC), Corniche alkhobar front(KBF), Half-Moon Beach (HMF), and Dammam corniche (DMC). In total,192 seawater samples were collected with measured physical parameters12such as pH, temperature, and TDS (total dissolved solids). Samples weretested to detect the presence of V. parahaemolyticus. IMB was used withTCBS and CHROMagar to increase the isolates of V. parahaemolyticus.Also, a molecular study was done by using PCR to confirm the identity of V.parahaemolyticus to the species level and identify virulence gene markers.ERIC PCR was performed to detect the similarities between V.parahaemolyticus strains. In total, 58 isolates were tested positive for V.parahaemolyticus after the use of TCBS and CHROMagar media with IMB,while the total number of isolates without IMB was only 9. To confirm theidentity of the species level, PCR targeting the toxR gene was applied and 28strains were detected. The pathogenicity of isolated strains was tested byusing PCR targeting tdh and trh genes, 23 isolates are tdh+ and trh- genesnot detected.Conclusion:The seawater samples were tested with and without IMB. The finding of thisstudy confirms the application of IMB in increase the detection level of V.parahaemolyticus. This study concludes that V. parahaemolyticus tdh+ ispresent in the analyzed samples of seawater collected from coastal areasalong the eastern coast of Saudi Arabia.1 0Item Unknown Childhood Psoriasis : A Clinical Study(Imam Abdulrahman Bin Faisal University, 1995) Al-Hadlaq, Abdulrahman; Aba-Hussain, Abdulaziz0 0Item Unknown Impetigo: A Clinical Study : In the Dermatology Clinic at King Fahd Hospital of the University(Imam Abdulrahman Bin Faisal University, 2003) Al-Ghamdi, Khalid Mohd; Abuhussain, Abdulaziz0 0Item Unknown Detection of Shiga Toxin Producing Escherichia coli O157:H7 by using Immunomagnetic Separation and PCR in Imported Frozen Beef Marketed in Eastern Province of Saudi Arabia(Saudi Digital Library) Almulhim, Ahlam FahadBackground: E. coli O157 is the most remarkable of the five known diarrheagenic E. coli (DEC). The global spread of E. coli O157:H7 among children has caused the Food and Agriculture Organization of the United Nations (FAO/UN) to improve standards for food security and quality. This bacterium is the most notorious because its potentially harbors virulence genes, which can result in kidney failure, particularly hemolytic uremic syndrome (HUS).Aims: This study aims to investigate the presence of E. coli O157 in imported frozen beef marketed in Saudi Arabia by using immunomagnetic beads, multiplex-PCR, and ERIC-PCR DNA fingerprinting.Methods: In this study, 201 imported frozen beef samples were purchased from different supermarkets in the Eastern Province of Saudi Arabia. All samples were analyzed by using immunomagnetic beads (IMB), CHROMagar O157, and SMAC agar. Virulence gene markers (stx-1, stx-2, and eae) were screened by using multiplex-PCR. ERIC-PCR DNA fingerprinting was used to identify the clonal relationship among the strains isolated from different countries.Results: The overall occurrence rate of presumptive E. coli was 43.8% in examined samples, obtained by using chromogenic agar without IMB and yielding 89 isolates. In chromogenic agar with IMB, the overall occurrence rate was 52.7% in examined samples from India, Australia, Brazil, and New Zealand, with a yield of 111 isolates. Three isolates were found to be positive for the stx-1 gene while no single isolate was detected for the stx-2 and eae genes among presumptive isolates that were recovered on SMAC agar without IMB. Meanwhile, on SMAC agar with IMB,xix8 isolates were found to be positive for the stx-1 gene in samples that were imported from Australia, India, and Brazil. No samples were positive for eae from New Zealand. The highest isolates harboring the stx-1 gene were detected in beef samples imported from Australia, for which the stx-1 gene was detected in 9 isolates out of 89 recovered on chromogenic agar without IMB. Two isolates were detected for the eae gene in beef samples imported from India, while no single isolate was detected for the stx-2 gene. The overall distribution rate of the stx-1 gene among the presumptive isolates of E. coli O157 which were examined from imported frozen beef were positive in 37 (12.4%) out of 298 isolates. The stx-2 gene was detected only in a single sample from Brazil and New Zealand. ERIC-PCR primers generated different DNA polymorphisms among 42 isolates of E. coli pathotypes and were able to group all confirmed 42 isolates of E. coli into 4 clusters (A, B, C, and D) by using the UPGMA algorithm and gel J software. Therefore, 85.7% of E. coli pathotype isolates were typeable with ERIC-PCR, while 14.3% produced a single cluster. ERIC-PCR was able to group all strains according to their countries of origin except for one isolate of Australian beef, which was grouped with E. coli isolates from Indian beef. In addition, one isolate of Brazilian beef was grouped with E. coli isolates from Australian beef.Conclusion: This study confirmed that the imported frozen beef marketed in the Eastern Province of Saudi Arabia was harboring virulence gene markers of E. coli O157. The used methods of IMB and multiplex-PCR proved to be more reliable and adequate for the detection of E. coli O157 in imported frozen beef. This study recommends that food authorities such as the Saudi Food and Drug Authority (SFDA) set adequate measures to monitor imported frozen beef.1 0