Imam Abdulrahman Bin Faisal University
Permanent URI for this collectionhttps://drepo.sdl.edu.sa/handle/20.500.14154/16
Browse
3 results
Search Results
Item Restricted Vitiligo in Childhood: A Clicial Study(Imam Abdulrahman Bin Faisal University, 1995) Shaikh, Yasir Hasan; Okoro, Anezi N0 0Item Restricted The Cellular and Molecular Impacts of Doxorubicin on Breast and Ovarian Cancer Cells(2022) ALKhalifah, Zahra Abdul Jaleel; ثابت، حسين حسن; المفتي، سارة أمينCancer is the most serious disease caused by gene mutation, these mutations can disturb three types of genes, DNA repair genes, proto-oncogenes, and tumor suppressor genes, which affect the cell cycle, proliferation, differentiation, and programmed cell death. Breast and ovarian cancer are the further most commonly occurring cancer in women globally. As of 2021, an estimated 281,550 females will be diagnosed with breast cancer, and about 43,600 of these females will pass away from this disease. Breast cancer (BC) may begin in various parts of the breast, such as the ducts, the lobules, or the tissues in between that contain many genetic and epigenetic abnormalities. Ovarian cancer (OC) has a high death rate worldwide and it is considered the seventh most fatal cancer among women globally. Cancer therapy has different approaches, chemotherapy is one of the best approaches, which is used worldwide. Doxorubicin (DOX) is one of the most effective chemotherapeutic drugs in cancer therapy. In the present study, we evaluated the role of DOX in cell cycle regulation, proliferation, differentiation, and apoptosis on BC, OC, and control healthy cells of human foreskin fibroblast cells. DOX was used in different doses (1 µM, 2 µM, 4 µM) and 0.1% DMSO for the control treatment, DOX incubation time was 24 h and 48 h. We performed a cell viability assay, wound healing assay, and quantitative real-time PCR. Finally, we evaluated the gene expression and alterations of different cancer-related genes. Results obtained indicated that there was suppression in some cancer-related genes (NOTCH1, PDGFA, TMEM45A, KRAS, NRAS, LAMA4, PTEN, ALK, and BRAF) in the MCF-7 and SKOV-3 cells, which indicate that the DOX has a major effect on inhibiting cancer cells proliferation.20 0Item Restricted Identifying Pathogenic Variations on Early Stage of Pregnancy Using Invasive and Non-Invasive Prenatal Samples(2022) Sonbol, Bayan Sameer Mohammed; Borgio, Francis; AbdulAzeez, SayedVarious factors can increase the risk of fetal genetic abnormalities during pregnancy, such as maternal age and family history. Prenatal diagnosis is a critical clinical practice for couples at risk of genetic disorders in the fetus. The study aims to identify pathogenic gene variations in the early stage of fetal development using invasive and non-invasive prenatal samples by developing fetal cell isolation, gender determination, and gene variation screening. Different samples were collected from various sources, maternal blood (n=10), serum (n=8), paternal blood (n=7), maternal buccal swab (n=8), maternal urine (n=9), and amniotic fluid (n=6). Coelomic fluid (n=1) was also collected for the first time in the history of Saudi Arabia. Different methods were used for fetal cell isolation: morphology based using a designed manual micromanipulator from invasive samples; Cluster of differentiation 71 (CD71) microbeads mediated magnetic cell sorting and percoll based subfractionation for fetal nucleated red blood cells (fNRBCs) from invasive and non-invasive samples. In-house multiplex gender PCR was used for the fetus gender determination. Nested and Amplification refractory mutation system (ARMS) PCR with newly designed primers carried out for detecting sickle cell disease (SCD) mutation (HBB:c.20A>T) in the isolated fetal cells. Our study has successfully identified a heterozygous sickle mutation in HBB gene from amniotic fluid cells and fetal DNA sample (n=3) using ARMS PCR. The study has also determined the fetal gender in single cell and fetal DNA using the gender PCR multiplex. All fetal DNA of fetal cells had a high accuracy than cell free fetal DNA (cffDNA). This study highlights that single-cell isolation from amniotic fluid and coelomic fluid could be used for accurate genetic screening for early diagnosis upon largescale validation.24 0