Saudi Cultural Missions Theses & Dissertations

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Isolation and characterization of bacteriophage in Providencia rettgeri.
(University of Manchester, 2024) Albalawi, Hind Faleh; Hargreaves, Kat
P. rettgeri is a gram-negative bacterium and a member of the Enterobacteriaceae family. It. an opportunistic pathogen, which can cause many infections but primarily urinary tract infections and is commonly associated with hospital-acquired infections. This bacterium is part of the natural microbiome found in the human gut. Also, is a ubiquitous bacterium that can be found in water sources, soil, and animals. The emergence of resistance in antibiotics in particular carbapenem is a significant concern. Also, it plays a role in the dissemination of this resistance across the world. Therefore, the exploration of alternative therapies is required. One of the most promising alternative therapies is bacteriophages. Hence, this study aims to isolate and characterize bacteriophages that target Providencia rettgeri. 12 environmental samples were collected from the outskirts of Manchester to isolate phages. In addition, bioinformatic tools analysis was used to predict and highlight the role of prophage in the bacteria genome. However, there were some challenges such as Phage induction and failure of phage propagation therefore, understanding and exploiting the P. rettgeri defence mechanism against phages is critical for further research.
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