Saudi Cultural Missions Theses & Dissertations
Permanent URI for this communityhttps://drepo.sdl.edu.sa/handle/20.500.14154/10
Browse
2 results
Search Results
Item Restricted Identifying the Therapeutic Application of Cold Atmospheric Plasma in Chronic Wound Healing: A Scoping Review(King's college london, 2024) Fallatah, Alaa; Dyson, AlexBackground: Cold atmospheric plasma (CAP) is a plasma that is generated through the application of electric or electromagnetic fields to a gas operates at a low temperature and is produced under atmospheric pressure, making it suitable for medical applications. It has demonstrated beneficial effects in various medical fields, including oncology, dentistry, and dermatology. Chronic wounds are a type of wounds that are difficult to heal, which can negatively impact individual quality of life, different treatment modalities are used to treat chronic wounds. However, due to the complexity of chronic wounds new therapies are required to address these complexities. CAP therapy has shown promising results in promoting wound healing. This review aims to assess these existing data on the application of CAP therapy in chronic wound healing. Objective: Explore the mechanisms by which Cold Atmospheric Plasma (CAP) therapy promotes chronic wound healing, assess its effectiveness across various chronic wound types, and identify the optimal treatment duration to balance both efficacy and safety in chronic wound care. Methods: A scoping review was conducted of English language articles published from 2019 onward that focus on the application of cold plasma therapy in chronic wound healing. A comprehensive literature search was performed across four databases—PubMed, Embase, Google Scholar, and ClinicalTrials.gov. Both clinical and pre-clinical studies were included. Articles were assessed for inclusion, and data were extracted from those deemed relevant. A thematic analysis was then conducted to identify recurring themes within the data. Results: Following the initial broad screening of relevant articles, 36 studies were selected for detailed analysis. The thematic analysis revealed six key themes, including patient characteristics, treatment outcome, patients-reported outcome, CAP therapy protocol, mechanisms of wound healing, and correlations each theme offers an insight into the use of cold plasma therapy for chronic wound healing. The data indicates that cold plasma therapy lacks follow-up studies, a lack of standardization in the application of cold plasma therapy for chronic wound healing, and an underrepresentation of various types of chronic wounds in the research. Conclusion: This review highlights the promising potential of cold plasma therapy (CAP) for chronic wound healing while identifying critical research gaps. Future research should address these gaps to better gauge the full therapeutic potential of CAP therapy in healing chronic wounds.20 0Item Restricted The effect of components of electrospun material on S. aureus, P. aeruginosa and E. coli bacterial growth and biofilm formation(Saudi Digital Library, 2023-11-28) Alomair, Munirah; Powell, LydiaThe prevalence of chronic wounds in immunocompromised patients has risen recently, leading to increased morbidity and mortality rates. The emergence of antibiotic resistance has necessitated novel technology for the treatment of these wounds. In order to provide a bespoke wound dressing for patients, Corryn Biotechnologies is developing an innovative device that electrospins a polymer solution directly onto a wound. The antibiotic activity of the electrospun components, namely dimethyl sulfoxide (DMSO), collagen 1, acetone and a non-disclosed polymer solution, were tested against gram-positive methicillin-resistant Staphylococcus aureus (MRSA 1004A) and gram-negative Pseudomonas aeruginosa PAO1 and Escherichia coli IR57 in both planktonic and biofilm assays to determine their effect on bacterial growth and biofilm formation. Minimum inhibitory concentration (MIC) assays were used to evaluate the effect of DMSO against MRSA 1004A, P. aeruginosa PAO1, and E. coli IR57, with resultant MICs of 25%, 6.25%, and 12.5%, respectively. The MICs of collagen 1 and acetone were >50% for both gram-positive and gram-negative bacteria. Confocal laser scanning microscopy (CLSM) revealed that DMSO significant inhibited the formation of MRSA 1004A biofilm biomass at both concentrations of 1/2 MIC DMSO (p<0.0001) and 1/4 MIC DMSO (p=0.0017), with increased cell death achieved with 1/2 MIC DMSO treatment (p=0.0003). The MRSA 1004A biofilm roughness significantly increased with 1/2 MIC DMSO (p=0.0008), but the average thickness area and average thickness biomass were significantly decreased (both p<0.0001) at 1/2 MIC DMSO treatment. The P. aeruginosa PAO1 biofilm biomass significantly increased with 1/2 MIC DMSO treatment (p=0.03), while cellular death decreased with 1/4 MIC DMSO treatment (p=0.038). The effects of the components of the electrospun solution against the most common strains of gram-positive and gram-negative bacteria in chronic wounds highlight the potential application of these components to form a bespoke wound dressing to reduce biofilm formation and aid in chronic wound healing.12 0