Saudi Cultural Missions Theses & Dissertations
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Item Restricted THE ANTIM ICROBIAL ACTIVITY OF COMMIPHORA MOLM OL (MYRRHA) EXTRACT(Long Island University, 0019-07-15) Alenezi, Tahrir; Bhattacharjee, MrinalThe Commiphoramolmol (myrrha) has been used as a traditional medicine for centuries in different cultures. An ethanol extract o f myrrha was evaporated under vacuum to obtain an oil. A 20% solution o f this oil in ethanol was used to determine antimicrobial activity against the Gram-positive bacteria S. aureus, the Gram-negative bacteria, E. coli, MVlONal, and fungi, (yeast), Saccharomyces cerevisiae. The MIC forÆ’. coli and S. aureus were determined on phosphate buffer since the oil did not show antibiotic activity on growing cells. The MIC o f myrrha oil in phosphate buffer for E. coli was 0.56% (5.6 mg/ml) and for S. aureus was 0.1% (1 mg/ml). However, the oil could be used to kill cells in a nutrient-rich medium provided growth o f the bacteria is first stopped using a bacteriostatic antibiotic such as Chloramphenicol. The results show that chloramphenicol enhanced the antimicrobial activity o f myrrha oil. Zone o f inhibition test shows myrrha extract has antibacterial property against S. aureus. Furthermore, the antifungal activity of myrrha extract was prodigious since most o f the cells were killed in 10 minutes at a dose o f 0.05% (0.5 mg/ml) o f myrrha extract. Repeated attempts to obtain an E. coli or S. aureus strain that is resistant to myrrha oil were unsuccessful. A possible explanation o f this can be that myrrha oil is a membrane acting antibiotic. In conclusion, the results o f this study suggest that myrrha extract could be a prom ising antibacterial and antifungal drug.33 0Item Restricted The effect of components of electrospun material on S. aureus, P. aeruginosa and E. coli bacterial growth and biofilm formation(Saudi Digital Library, 2023-11-28) Alomair, Munirah; Powell, LydiaThe prevalence of chronic wounds in immunocompromised patients has risen recently, leading to increased morbidity and mortality rates. The emergence of antibiotic resistance has necessitated novel technology for the treatment of these wounds. In order to provide a bespoke wound dressing for patients, Corryn Biotechnologies is developing an innovative device that electrospins a polymer solution directly onto a wound. The antibiotic activity of the electrospun components, namely dimethyl sulfoxide (DMSO), collagen 1, acetone and a non-disclosed polymer solution, were tested against gram-positive methicillin-resistant Staphylococcus aureus (MRSA 1004A) and gram-negative Pseudomonas aeruginosa PAO1 and Escherichia coli IR57 in both planktonic and biofilm assays to determine their effect on bacterial growth and biofilm formation. Minimum inhibitory concentration (MIC) assays were used to evaluate the effect of DMSO against MRSA 1004A, P. aeruginosa PAO1, and E. coli IR57, with resultant MICs of 25%, 6.25%, and 12.5%, respectively. The MICs of collagen 1 and acetone were >50% for both gram-positive and gram-negative bacteria. Confocal laser scanning microscopy (CLSM) revealed that DMSO significant inhibited the formation of MRSA 1004A biofilm biomass at both concentrations of 1/2 MIC DMSO (p<0.0001) and 1/4 MIC DMSO (p=0.0017), with increased cell death achieved with 1/2 MIC DMSO treatment (p=0.0003). The MRSA 1004A biofilm roughness significantly increased with 1/2 MIC DMSO (p=0.0008), but the average thickness area and average thickness biomass were significantly decreased (both p<0.0001) at 1/2 MIC DMSO treatment. The P. aeruginosa PAO1 biofilm biomass significantly increased with 1/2 MIC DMSO treatment (p=0.03), while cellular death decreased with 1/4 MIC DMSO treatment (p=0.038). The effects of the components of the electrospun solution against the most common strains of gram-positive and gram-negative bacteria in chronic wounds highlight the potential application of these components to form a bespoke wound dressing to reduce biofilm formation and aid in chronic wound healing.12 0