Saudi Cultural Missions Theses & Dissertations
Permanent URI for this communityhttps://drepo.sdl.edu.sa/handle/20.500.14154/10
Browse
2 results
Search Results
Item Restricted In-Vitro Effects of Recombinant IL-37 protein on Bladder cancer cell line Growth and Proliferation(University of East London, 2024-08) Alamri, Ashwaq; Gobena, EdessaIntroduction: Interleukin-37 (IL-37) is a cytokine of the IL-1 family, recognized for its anti-inflammatory and immunomodulatory functions. Despite growing interest in IL-37’s role in different types of cancers, its impact on bladder cancer growth and proliferation has not been thoroughly investigated. This study aims to explore the effects of recombinant IL-37 protein on the viability, proliferation, and apoptosis of T24 bladder cancer cell lines, addressing a significant gap in current cancer research. Methods: In this in vitro study, T24 bladder cancer cells were treated with different concentrations of recombinant IL-37 protein (1 μg/ml, 0.5 μg/ml, and 0.25 μg/ml) with negative control (0 μg/ml). Cell viability and apoptosis were assessed using flow cytometry, and the levels of pro- inflammatory cytokines IL-6 and IL-8 in the cell culture supernatants were quantified using enzyme-linked immunosorbent assay (ELISA). Statistical analysis was performed using one-way ANOVA followed by Tukey’s post hoc test to determine the significance of the results. Results: The results showed that IL-37 induces apoptosis in a dose-dependent manner, with the highest concentration (1 μg/ml) significantly reducing the percentage of viable cells from 90% to 71% and increasing the apoptotic cell population from 7% to 27%. Additionally, IL-37 treatment modulated the secretion of IL-6 and IL-8, with a significant increase in IL-6 production at 0.25 μg/ml (P ≤ 0.05) compared to the control. However, IL-37 did not significantly inhibit IL-8 production across the different treatment groups. (P ≤ 0.05). Conclusion: This study demonstrates that IL-37 not only promotes apoptosis in bladder cancer cells but also alters the tumor microenvironment by modulating cytokine secretion. These findings suggest that IL-37 may hold therapeutic potential as a novel approach to bladder cancer treatment. Further research is necessary to validate these results in vivo and to elucidate the precise molecular mechanisms through which IL-37 exerts its anti-tumor effects.16 0Item Restricted IL36 and IL37Cytokines, Mediators or Potential Modulators of Airway Infection and Inflammation?(Saudi Digital Library, 2022-10-01) Alshammari, Jamilah; Flannagan, BrianRespiratory tract infections caused by viruses or bacteria are among the most common human diseases worldwide. More specifically, respiratory syncytial virus (RSV) is a major cause of acute lower respiratory infection (ALRI), and this frequently results in hospitalisation in young children, particularly among those under the age of 5, and the elderly. Infection with RSV is also correlated with various airway respiratory diseases, such as bronchiolitis and asthma. In vivo testing suggests that levels of IL-36 alpha, IL-36 gamma, and IL-37 protein can be very high in nasopharyngeal aspirate (NPA) during RSV and rhinovirus (RV) infections. This work thus hypothesises that, during RSV and RV infections, the epithelial cells in the airways may express IL-36α, IL-36γ, and their respective receptors in a manner that plays a crucial pro-inflammatory role in RSV and RV infections. In addition, this work hypothesises the possibility of the further expression of IL-36Rα and IL-37 cytokines by airway epithelial cells (AECs) in response to RSV and RV infections. The main aim of this work, as described in this thesis, is thus to investigate whether RSV infection can trigger AECs to express IL-36 and IL-37 cytokines and their receptors.2 0