Dissecting Symbiosis Permissiveness in Parasponia andersonii

dc.contributor.advisorGeurts, Rene
dc.contributor.authorAlhusayni, Sultan Awwadh Sultan
dc.date.accessioned2024-12-23T05:23:52Z
dc.date.issued2024-12-17
dc.description.abstractNutrient limitations often constrain plant growth, leading to the use of chemical fertilizers, which can harm the environment. Biological nitrogen fixation, involving nitrogen-fixing bacteria in plant roots, provides a sustainable alternative. My research aligns with this goal, focusing on non-legume plants, particularly the non-nodulating Trema orientalis RG33 and the nodulating Parasponia andersonii species. One key gene I investigated was SYMRK, essential for root nodules and AM symbioses in legumes. In T. orientalis RG33, I identified a point mutation in this gene, and when introduced into the P. andersonii SYMRK gene, it did not disrupt the gene's function, successfully restoring both root nodule formation and AM symbiosis in a P. andersonii symrk mutant. I also explored the ectopic expression of NSP2, which enhanced AM mycorrhization but inhibited nodulation in Parasponia. Lastly, I discovered that the autoregulation of nodulation (AON) pathway, which regulates nodule numbers, is conserved in both legumes and non-legumes, providing a promising strategy for engineering nitrogen fixation in non-nodulating plants.
dc.format.extent254
dc.identifier.isbn978-94-6510-244-3
dc.identifier.urihttps://hdl.handle.net/20.500.14154/74366
dc.language.isoen
dc.publisherWageningen University and Research
dc.subjectSymbiosis
dc.subjectParasponia andersonii
dc.subjectNodulation
dc.subjectMycorrhization
dc.titleDissecting Symbiosis Permissiveness in Parasponia andersonii
dc.typeThesis
sdl.degree.departmentExperimental Plant Sciences
sdl.degree.disciplineBiology
sdl.degree.grantorWageningen University and Research
sdl.degree.nameDoctor of Philosophy

Files

Original bundle

Now showing 1 - 1 of 1
No Thumbnail Available
Name:
SACM-Dissertation.pdf
Size:
13.19 MB
Format:
Adobe Portable Document Format

License bundle

Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.61 KB
Format:
Item-specific license agreed to upon submission
Description:

Copyright owned by the Saudi Digital Library (SDL) © 2024