Evaluation of the effectiveness of SMI in the diagnosis of wound

Abstract

There has been great research interest on anaerobic organisms in polymicrobial infection, particularly post-surgical wounds, skin and soft tissue infections. Many studies have explored an effective conventional diagnostic method, as anaerobes are difficult to detect and deal with in clinical laboratories. This project uses selective media as an alternative method of isolation and identification of anaerobes. Eight types of anaerobic selective media were quantitively analysed to determine their effectiveness in Bacteroides fragilis and Clostridium sporogenes isolation. The efficacy of those media was achieved using the Miles-Misra technique with anaerobic condition optimisation then tested them in mixed culture using wound samples. The result showed that nearly all of the selective media are effective and can be used to isolate anaerobic bacteria within 24 hours. This approach can support the growth of low anaerobe quantity that might be present in a wound sample by using a combination of two or three types of selective media, which may then lead to early diagnosis and treatment. This makes the method beneficial for the improvement of diagnostic laboratories by reducing the turnaround time of the final report.