Promoter Testing for Plant Transgene Expression of High Value Proteins of Industrial Interest

Abstract

Abstract This study investigates the efficacy of various promoter-terminator combinations in Nicotiana benthamiana to enhance gene expression, which is crucial for advancements in plant synthetic biology. Utilizing green fluorescent protein (GFP) as a reporter, we analyzed the transient expression from multiple constructs: plasmids X12 (CaMV 35S double promoter with AtAct2 terminator), X52 (SlRbcS2 promoter with AtAct2 terminator), X64 (AtAct2 promoter with StATPase terminator), X65 (SlRbcS3A promoter with AtGu7 terminator), X67 (SlRbcS3A promoter with AtuNos terminator), X19 (STLS promoter with AtuNos terminator), JB-1 and JB-2 (both with 35S long promoter and AtuNos terminator). Each construct was assembled using Agrobacterium-mediated transformation for in vivo testing. Our results highlight the differential impacts of these combinations on GFP expression levels, suggesting that specific promoter-terminator pairings can significantly enhance or lessen gene expression. These findings offer valuable insights into the design of genetic constructs for improved performance in plant.