Cell penetrating Peptide-Drug-Conjugates (PDC) in the delivery of FITC-G3 labelled and siRNA for breast cancer cancer cells therapy
Abstract
The cell-penetrating peptides development in drug delivery is an auspicious method for confronting diseases like cancer. Thus, it is important to not just develop new drug conjugated vectors containing high specificity to tumors’ conditions, but to gain insight on their intercellular fate and their biological internalization mechanisms as well. Antimicrobial peptides have the capacity to eradicate pathogen microorganisms, on the contrary they have been applied as conjugates alongside nucleic acids or alternative active molecules for the intended cell delivery. The FITC-labelled G3 represents a cell-penetrating peptide which has been recognized for its specificity in penetrating cancer cells through a clathrin-mediated endocytosis, though the biological machinery which participates in the process is yet to be completely explained. Therefore, this project’s principal objective was to evaluate whether the G3 in MDAmb231 cells’ cellular uptake mechanism was receptor-dependent. In the cellular uptake mechanism for numerous cell-penetrating peptides, Scavenger receptors have been recognized as indispensable proteins. Accordingly, the scavenger receptor superfamily’s gene library knockdown was conducted in the identification of the key receptor interactions within the internalization trail. Alternatively, the results obtained from the high-content targeted library screen, proposes that the subsequent scavenger receptors may generate primary proteins for the FITC-G3 penetration into breast cancer cells. Conclusively, this information on the cellular internalization route for G3 mediated through scavenger receptors, is a step forward for their future use in the cancer therapy