The Role of DNA Repair Pathways in the Resistance to Nucleoside Analogue Cytarabine

Abstract

Cytarabine is a treatment of haematological malignancies which inhibits DNA synthesis. In the current project the aim is to identify whether Cytarabine integrates into the DNA and explore different mechanisms that might contribute to Cytarabine removal. However, XTT assay was used to illustrate the role of MRE11 and Polε nuclease activity in Cytarabine resistance and removal. In addition, mass spectrometry was used to identify whether Ara-C integrated into the DNA and the amount of replication both with and without cytarabine.