Effects of Probiotic Lactobacilli on Streptococcus mutans: Implications for Early Childhood Caries
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Date
2025-06
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Publisher
Saudi Digital Library
Abstract
Effects of Probiotic Lactobacilli on Streptococcus mutans:
Implications for Early Childhood Caries
WALAA MOHIEDEN AL TURKI
DENTISTRY
ABSTRACT
Purpose:
Probiotic Lactobacillus species have been shown to have beneficial effects against early
childhood caries (ECC) by inhibiting the growth of cariogenic bacteria. However, the
acidogenicity of Lactobacillus spp. could have potential carcinogenic effect. The objective of this
study was to investigate the effects of probiotic lactobacilli and cariogenic lactobacilli on the
growth, biofilm formation, and acid production of Streptococcus mutans (S. mutans).
Methods:
Growth competition between probiotic L. rhamnosus GG (L-GG) or caries-associated L.
rhamnosus M72-26 (L-M72) and S. mutans UA159 were assessed on Todd Hewitt Yeast (THY)
agar plates and De Man-Rogosa-Sharpe (MRS) agar plates. Lactobacilli and S. mutans single- and
dual-species biofilm formation in THY and MRS media containing 1% sucrose were evaluated via
crystal violet staining. Acid production by lactobacilli and S. mutans single- and dual-species
biofilms at different time intervals were analyzed using an Orion pH meter.
Results:
There was no inhibition of S. mutans growth by L-GG or L-M72 on THY agar plates;
inhibition was noted only on MRS agar plates. L-GG was able to reduce S. mutans biofilm
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formation in direct assay but did not inhibit the biofilm formation in the indirect assay. In addition,
the dual-species biofilm showed similar levels of acid production as lactobacilli single-species
biofilms.
Conclusions:
Probiotic lactobacilli may prevent ECC development by inhibiting the growth and biofilm
formation of S. mutans only under favorable conditions. Further studies will evaluate the in vivo
effects of L-GG on S. mutans-induced caries development in animal models.
Description
Keywords
Probiotic, Lactobacilli, Streptococcus mutans, Early Childhood Caries, Biofilm, Acidogenicity