ارتباط تعدد أشكال جين برونايز سيرين rs4820268 (TMPRsS6) مع مرضى فقر دم نقص الحديد في منطة جازان

Abstract

Background: Iron deficiency anemia (IDA) is one of the most prevalent diseases globally affecting people of all ages. It is characterized by reduced red blood cells, hemoglobin, and serum iron. Several investigations have established links between specific SNPs within the TMPRSS6 gene and various iron-related characteristics. This study aimed to investigate the prevalence of rs4820268 polymorphism in Jazan region and its association with anemia. Methods: A cross-sectional study included 60 males and 114 females participants from Jazan University was carried out. Five ml of EDTA and plain tube venous blood samples collected for CBC and Biochemical parameters studies, respectively. The genomic DNA was extracted from the peripheral blood sample and genotyping of rs4820268 was performed using designed ARMS-PCR primers. Results: The Results revealed that 94.5% (n = 52) of male students had normal levels of hemoglobin (Hb), whereas only 5.5% (n = 3) were found to have anemia. Conversely, within the female group, 39.1% of subjects exhibited a low concentration of Hb. The most predominant genotype in the cohort study was CC followed by CT genotypes. The binary logistic regression analysis revealed that there was no statistically significant association between rs4820268 genotypes and the risk of developing anemia. Conclusion: This study demonstrates that the rs4820268 CC genotype is more prevalent in Jazan region but without any association on the development of anemia. More studies are needed with a larger and more diverse sample size to explore the potential impact of rs4820268 polymorphism on anemia.