DNA REPAIR IN THE SULFOLOBUS ARCHEON

Abstract

Objective: The purpose of this study was to identify putative operons in the Sulfolobus archaean genus and explore the role of its genes in DNA repair mechanisms. By doing so, we aim to contribute to the understanding of the complex processes involved in DNA repair in these organisms and to conduct a comparative study of the operons containing Mre11, Rad50 (SbcC, SbcD), HerA, and NurA across the archaea and bacteria domains by studying the genes occurring alongside these four primary genes in the operons across different species. Methods: To search for putative operons, we used a combination of bioinformatics tools (Terminal, Python scripts and Gephi program) and experimental techniques (MicrobesOnline software, Blast, NCBI). We first conducted a genome-wide search for genes involved in DNA repair and then analysed their genomic context to identify potential operons. Results: Our analysis identified several putative operons involved in DNA repair in Sulfolobus and bacteria species. These operons are highly conserved across different strains of Sulfolobus and bacteria and are involved in a variety of DNA repair mechanisms, including base excision repair, nucleotide excision repair, and homologous recombination. Our experiments also confirmed the function of these operons in DNA repair and provided insights into their regulation and interaction with other repair pathways. Conclusion: Our study provides a comprehensive overview of DNA repair mechanisms in the Sulfolobus archaean genus and many bacterial species and sheds light on the role of putative operons in these processes. Our findings have important implications for the field of bioinformatics and genetic research as they provide a foundation for further studies on DNA repair mechanisms in Sulfolobus and other organisms. Keywords: DNA repair, Sulfolobus, operons, bioinformatics, genetic research, Mre11, Rad50, NurA, HerA, bacteria species, thermophilic, mesophilic, putative operon, MicrobesOnline