SACM - Germany
Permanent URI for this collectionhttps://drepo.sdl.edu.sa/handle/20.500.14154/9655
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Item Restricted Comparing human fetal liver-derived mesenchymal stromal cells to human adult bone marrow-derived mesenchymal stromal cells(Saudi Digital Library, 2019) Toonsi, Mawaddah; Götherström, Cecilia; Abomaray, Fawaz; Walther Jallow, LilianBackground: Mesenchymal stromal cells (MSC) can be isolated from various tissue sources and can be derived from adult and fetal tissues. Depending on their age, MSC have different therapeutic potentials. In this study human fetal liver MSC (fMSC) were compared to adult bone marrow MSC (aMSC) regarding their phenotype and function. Methods: fMSC and aMSC were compared in terms of their size using CASY TT and their senescence using ß-galactosidase staining. Population doublings (PD) and population doubling time (PDT) were also examined. Following induction of osteogenic differentiation, calcium deposits were stained using Alizarin red S. Finally, flow cytometry was used to examine the cells’ phenotype and purity. Results: fMSC had a significantly lower peak diameter compared to aMSC. Moreover, fMSC displayed a greater number of PD and a shorter PDT in comparison to aMSC, and the percentage of fMSC undergoing senescence was significantly lower than aMSC. Furthermore, fMSC that were induced into osteoblasts deposited more calcium salts, and hence stained more positive for Alizarin red S relative to aMSC. Finally, both cell sources were positive for the MSC markers CD90 and CD73 and negative for the non-MSC markers CD45, CD31 and HLA class II. Conclusions: The results of the study demonstrate that fMSC possess a greater proliferative and osteogenic differentiation capacity compared to aMSC. Altogether, fMSC may be a more robust cell source compared to aMSC as a therapy for diseases, including those that require bone repair.13 0Item Restricted THE EFFECT OF PORCELAIN THICKNESS IN RELATION TO PREPARATION DEPTH ON FRACTURE STRENGTH OF TRANSLUCENT ZIRCONIA CROWNS(Malmo University, 2016) Bakitian, Fahad; Steyern, Per Vult vonObjective. The purpose of the study was to evaluate the fracture strength of veneered high translucent Y-TZP crowns with the same outer dimensions, but with different porcelain layer thicknesses, with different inner dimensions (preparation depths). Material and methods. Seventy standardized crowns divided into seven groups (n = 10) were fabricated. The crowns in the first six groups were divided according to different thicknesses of the porcelain layer on Y-TZP copings: 0.3 mm, 0.5 mm, 0.8 mm, 1.0 mm, 2.0 mm, and 2.5 mm. The crowns in the seventh group had the same porcelain thickness as group 1 but with preparation depth as group 6. All crowns underwent thermocycling, storing in moist environment and cyclic pre-loading. After fatigue, the crowns underwent to single-load-to-failure test. Results. The crowns with 1.0, 0.8 and 0.5 mm of porcelain layer thicknesses showed no significant differences in fracture strength (1540 N, 1313 N, and 1286 N). Groups 2.5 mm and 2.0 mm showed significantly lower fracture strength (851 N and 910 N) compared to all other groups. There was no statistical difference in fracture strength of crowns with same thickness but with different preparation depths (1136 N and 1202 N). Conclusions. The reduction in porcelain thickness to 0.5 mm does not impair the fracture strength of crowns significantly. Fracture strength of crowns with micro-coating layer of porcelain (0.3 mm) might be lower, but still in range of what is considered to be clinically sufficient. Preparation depth (die size) do not affect the fracture strength.6 0