Imam Abdulrahman Bin Faisal University
Permanent URI for this collectionhttps://drepo.sdl.edu.sa/handle/20.500.14154/16
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Item Restricted Phenotypic and Genotypic Characterization of von Willebrand Factor Gene of Saudi Healthy Individuals in Eastern Province of Saudi Arabia(Imam Abdulrahman Bin Faisal University, 2020) Aldossary, Nemat Abdullah Ahmed; Gameel, Fathelrahman Mahdi HassanBackground:Vibrio parahaemolyticus is a gram-negative, facultative, anaerobic,halophilic bacterium that is widely disseminated in coastal, estuarine, andmarine environments. It is a seafood-borne pathogen that, in humans, cancause gastroenteritis. There are many clinical symptoms, such as diarrhea,abdominal pain, fever, and vomiting. Not all strains of V. parahaemolyticusare pathogenic, except, those are expressed tdh or trh genes. The polymerasechain reaction is the most popular molecular technique used to detect V.parahaemolyticus virulence genes. Recently, many studies have used anewly established technique known as immunomagnetic beads (IMB) toconcentrate the bacterium. This will increase the possibility of detecting V.parahaemolyticus from environmental, food, and clinical samples. Thisstudy aims to use PCR and IMB to detect V. parahaemolyticus in coastalwater samples.Method and result:From February 2018 to May 2018, seawater samples were collected fromfive different areas along the Arabian Gulf Coast in Saudi Arabia, includingAziziya Beach (AZB), Corniche alkhobar (KBC), Corniche alkhobar front(KBF), Half-Moon Beach (HMF), and Dammam corniche (DMC). In total,192 seawater samples were collected with measured physical parameters12such as pH, temperature, and TDS (total dissolved solids). Samples weretested to detect the presence of V. parahaemolyticus. IMB was used withTCBS and CHROMagar to increase the isolates of V. parahaemolyticus.Also, a molecular study was done by using PCR to confirm the identity of V.parahaemolyticus to the species level and identify virulence gene markers.ERIC PCR was performed to detect the similarities between V.parahaemolyticus strains. In total, 58 isolates were tested positive for V.parahaemolyticus after the use of TCBS and CHROMagar media with IMB,while the total number of isolates without IMB was only 9. To confirm theidentity of the species level, PCR targeting the toxR gene was applied and 28strains were detected. The pathogenicity of isolated strains was tested byusing PCR targeting tdh and trh genes, 23 isolates are tdh+ and trh- genesnot detected.Conclusion:The seawater samples were tested with and without IMB. The finding of thisstudy confirms the application of IMB in increase the detection level of V.parahaemolyticus. This study concludes that V. parahaemolyticus tdh+ ispresent in the analyzed samples of seawater collected from coastal areasalong the eastern coast of Saudi Arabia.3 0Item Restricted Phenotypic and genotypic characterization of patients diagnosed with Von Willebrand Disease type 1 in the eastern province in Saudi Arabia(Imam Abdulrahman Bin Faisal University, 2020) Al Faris, Asma Abdulrazaq Faris; Albashawri, LaylaBackground: Venous thromboembolism (VTE) is one of the major complications inmost cancer patients leading to poor prognosis and short survival. Several commonclinical risk factors coexist in cancer patients are used as risk predictive biomarkers tohelp in the management and prevention of VTE. These include cancer site and stage,chemotherapy regimen and elevated biological markers. However, Geneticpolymorphisms in genes controlling coagulation and fibrinolysis significantly associatedwith VTE if detected, then they might be more sensitive individual predictive biomarkersfor VTE risk assessment. This study was conducted to evaluate the association betweenITGB3 rs3809865 and rs5918 with VTE risk as well as monitor the effect of VTE onoverall survival of these cancer patients.Method: In this retrospective case-control study, 195 FFPE cancer patients’ sampleswere collected (controls n=157, case n=38) using the stored data through Jan 2010 to Sep2018 from King Fahad Specialist Hospital in Dammam. Samples were genotyped usingTaqMan genotyping assay, then logistic regression analysis and Chi-square were used topredict the association between risk factors and VTE. Survival Comparison was tested bythe log-rank test.Results: Genetic polymorphisms in ITGB3 (rs3809656 and rs5918) found not to beassociated with VTE increasing risk in cancer patients (P>0.05). While the advancedstage was potentially increasing the risk of VTE events (OR 5.1 CI 2.01-12.9 P=0.001).Patients with VTE showed a poor overall survival reflected by the median survival rateof only three years compared to seven years for cancer patients without VTE.Conclusion: This study highlighted the potential influence of VTE on prognosis andsurvival of cancer patients and raised the importance of exploring risk predictivebiomarkers in our population. This will improve the risk prediction biomarkers leadingto implementing safe and effective thrombosis prophylaxis strategies7 0