Saudi Cultural Missions Theses & Dissertations
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Item Restricted INJECTION OF RAT MESENCHYMAL STEM CELLS LEADS TO HOMING AND DIFFERENTIATION IN THE LIVER IN A BLUNT LIVER TRAUMA MODEL(Saudi Digital Library, 2020-12-02) Alhabboubi, Mostafa; Razek, Tarek; Shum Tim, Dominique; Gao, Zu Ha; Khwaja, Kosar; Khwaja, KosarBackground: The liver heals remarkably after different forms of injuries. However, healing time can be lengthy following high-grade blunt injuries. We hypothesize that injected bone marrow derived mesenchymal stem cells (MSC) could locate and differentiate to hepatocytes after blunt trauma using a rat liver trauma model. Methods: Blunt liver trauma was induced to Lewis rats. MSC were extracted from Lewis rats’ femurs and transfected with LacZ retrovirus so that they express B- galactosidase enzyme, giving their nuclei a blue color on light microscopy. Each rat received a single dose of BMDS (n=6 x 106) within 24 hours of trauma. Through different steps of the experiment, route of injection was the tail vein (TV) in 9 rats, the portal vein (PV) in 19 rats and directly to the injured liver (DI) in 6 rats. Rats were euthanized at 2, 7 days after injection of MSC. Livers were harvested and examined under light microscopy to identify the MSC. Results: Liver sections showed localization and active migration of MSC to trauma sites in the PV group euthanized at 48 hours (3/10 rats). Furthermore, some stem cells differentiated to hepatocytes. Although with fewer cells, similar findings were present in 1/9 rats euthanized at 7 days in the PV group. There was no evidence of MSC localization in TV and DI groups. 4 Conclusions: MSC can locate and differentiate to hepatocytes at blunt trauma site and may contribute to liver regeneration process. Portal vein injection of MSC has emerged as the most effective method of delivery to the liver following trauma among different delivery methods studied. This technique has the potential to become an effective therapeutic strategy to improve liver regeneration after severe blunt trauma. Methods of optimizing homing to injured tissue and evaluation of differentiated stem cell functionality are future areas of research.3 0Item Restricted Development of a core outcome set for lower limb orthopaedic suregical interventions in ambulant children and young people with cerebral palsy(2023-05-09) Almoajil, Hajar; Dawes, Helen; Toye, Francine; Theologis, Tim; Hopewell, Sally; Saboskbar, AfsieFirst and foremost, I offer my sincerest gratitude to my primary supervisor, Mr. Tim Theologis, for his guidance and support, valuable advice and feedback, for being straight-forward, for not giving up and patiently helping me to stay on the track and unwavering support, in many an hour of self-doubt. I would like to thank him for seeing the potential and believing in me, encouraging me to pursue this body of work. Thank you! I am grateful to my co-supervisors, Professor Helen Dawes, Professor Sally Hopewell and Dr Francine Toye, for offering their expertise, insight, and for their patience, guidance. Thanks must go to Professor Crispin Jenkinson, for his support and contribution and guidance on setting up the project. I am extremely grateful to all advice from members of our Patient and public Involvement group, and the steering committee, ensured that this work focussed on what mattered most to children undergoing lower limb orthopaedic surgery. This project, which I am, always have and always will be passionate about, would not have been possible without the participation of children and young adults with CP, their families and healthcare professionals across the UK and colleagues internationally, who have participated with excitement, wisdom and honesty. I hope that the influences of this research are far-reaching. I am indebted to the Imam Abdulrahman bin Faisal University for funding me to undertake a DPhil program. This decision has made an incredibly positive impact on my life, and I hope, produced work that will benefit children with CP internationally. To my colleagues, Foteini Mavrommati, Stella Koukides, Jerneja Uhan, Norah Almulhem and Wafa Almusallam for their guidance and inspiring me every single time we met through the years in completing this research. I reserve my most heartfelt thanks for my family. My parents and family, for their constant love and support and for believing in me enthusiastically. Most of all, I would like to thank my husband, Abdullah, who has been my biggest supporter, and whose bravery, integrity and resilience are a constant source of inspiration.27 0